双丹明目胶囊对糖尿病大鼠模型视网膜VEGF-a、VEGF-b、VEGF-c及 其受体Flk-1表达的影响
Effects of Shuang Dan Ming Mu Capsule on Expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, in Diabetic Retinopathy Rats
摘要
Abstract
Objective The effects of Shuang Dan Ming Mu capsule on expression of VEGF-a, VEGF-b, VEGF-c and the VEGF receptor, Flk-1, were examined in a diabetic retinopathy rats model. Methods Forty Sprague-Dawley (SD) rats were randomized into Groups A (blank), B (model), C (Shuang Dan Ming Mu) and D (positive control) group, with each group containing 10 rats and 20 eyes. Rats from groups B, C and D were administered one dose of 50 mg/kg streptozotocin (STZ) by tail vein injection to establish a diabetic rat model. One week after model preparation, medication was continuously administered by gavage. After gavage for 8 weeks, the animals were sacrificed and retinal expression of VEGF-a, VEGF-b, VEGF-c and Flk-1 was quantified by immunohistochemical analysis. Results At week 8 of drug administration after model preparation, the average protein expression grayscale values for VEGF-a, VEGF-b, VEGF-c and Flk-1 in the rats model, Shuang Dan Ming Mu and positive control groups were all lower than those in the normal group, while the mean optical density values were higher than those in the normal group. When the model group was compared to the normal group, the difference was extremely significant (P < 0.01). The mean grayscale values of VEGF-a, VEGF-b, VEGF-c and Flk-1 in the Shuang Dan Ming Mu and positive control groups were all higher than those in the model group, while the mean optical density values were lower than those in the model group (P <0.05 or 0.01). Conclusion Shuang Dan Ming Mu capsule can significantly decrease the expression of VEGF-a, VEGF-b, VEGF-c and Flk-1 in the retinas of diabetic model rats and exhibit some protective effects in their retinas.关键词
双丹明目胶囊/糖尿病大鼠模型/VEGF-a/VEGF-b/VEGF-c/Flk-1Key words
Shuang Dan Ming Mu capsule/Diabetic rats model/VEGF-a/VEGF-b/VEGF-c/Flk-1引用本文复制引用
彭俊,潘坤,刘峥嵘,秦裕辉,彭清华..双丹明目胶囊对糖尿病大鼠模型视网膜VEGF-a、VEGF-b、VEGF-c及 其受体Flk-1表达的影响[J].数字中医药（英文）,2018,1(3):228-238,11.基金项目
We thank for the funding support from the National Natural Science Foundation General Program (No. 814737370) （No. 814737370）
Hunan Province Graduate Student Research Innovation Program (No. CX2016B377 and No. CX2017B432) （No. CX2016B377 and No. CX2017B432）
Traditional Chinese Medicine Prevention and Treatment of Facial Feature Disease Hunan Province Key Laboratory Construction Program (No. 2017TP1018) （No. 2017TP1018）
Changsha City Science and Technology Program (No. kc1704005) （No. kc1704005）
Central Finance Supported Local High School Construction Project （）
State Administration of Traditional Chinese Medicine Ophthalmology Key Discipline Construction Project （）
Hunan Province Traditional Chinese Medicine Facial Feature Key Discipline Construction Project. （）
