数字中医药（英文）2018，Vol.1Issue(4)：261-271,11.
天然牛黄、体外培育牛黄和人工牛黄中胆汁酸类成分及其在人肠源Caco-2细胞模型中吸收转运的对比研究
Quantitative Comparison of Bile Acid Distribution and Intestinal Transport from Native Cow-bezoar and Artificial and in vitro Cultured Substitutes using Caco-2 Cell Monolayer Model
摘要
Abstract
Objective This study was conducted to examine the absorption and translocation of conjugated bile acids (BAs) in Calculus bovis and its substitutes to detect differences in these materials. Methods A Caco-2 monolayer cell model was used to compare the apparent permeability coefficient (Papp) value and efflux ratio (ER) of BAs in natural cow-bezoar (NCB), artificial cow-bezoar (ACB), and in vitro cultured cow-bezoar (Ivt-CCB). Papp and ER values were determined by liquid chromatography-mass spectrometry. Samples were separated on an analytical column. Results The distribution of BAs in NCB was significantly different from that in ACB and Ivt-CCB. The percentages of conjugated BAs were significantly higher in NCB than in the two substitutes. The distribution differences of conjugated and unconjugated BAs can be used to distinguish costly NCB from relatively inexpensive substitutes. Conclusion The transport characteristics of BAs in Ivt-CCB were more consistent with NCB than with ACB, even when the proportions of BAs in Ivt-CCB were closer to those of ACB.关键词
胆酸/牛黄/肠转运/Caco-2细胞/表观通透系数/外排率Key words
Bile acids/Cow-bezoar/Intestinal transport/Caco-2 cell/Apparent permeability coefficient value/Efflux ratio引用本文复制引用
陈颖,胡晓茹,程显隆,马双成,冉庆森,杨庆,强伟杰,戴忠,朱晓新..天然牛黄、体外培育牛黄和人工牛黄中胆汁酸类成分及其在人肠源Caco-2细胞模型中吸收转运的对比研究[J].数字中医药（英文）,2018,1(4):261-271,11.基金项目
We thank for the funding support from the Youth Development Research Foundation of NIFDC (2014A1), National Science and Technology Major Project (2017ZX09101002-002-008), and the National Natural Science Foundation of China (No. 81403171 and No. 81603517). （2014A1）
