基于生物信息学整合分析探讨青光眼高压反应性视神经星形胶质细胞相关核心基因
Hub Genes of Astrocyte Involved in Glaucoma with Ocular Hypertension by Integrated Bioinformatics Analysis
摘要
Abstract
Objective This study was conducted to elucidate the potential key candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension. Methods Expression profiles GSE2378 and GSE758 including 27 reactive optic nerve head astrocytes (ONHAs) by hypertensions and 26 normal controls, were integrated and deeply analyzed. Differentially expressed genes (DEGs) were sorted and candidate genes and pathways enrichment were analyzed. DEGs-associated protein-protein interaction network (PPI) was performed. Results A total of 119 consistently expressed genes were identified from 281 commonly changed DEGs, including 68 up-regulated genes and 51 down-regulated genes. PPI network complex filtered 75 DEGs (43 up-regulated and 32 down-regulated genes) of the 119 consistently altered DEGs and developed 117 edges, and 10 hub genes were identified. The most significant 3 modules were filtered from PPI, pathway enrichment analysis showed that module 1 was associated with extracellular exosome. Module 2 was mainly associated with antibody-dependent cellular cytotoxicity (ADCC) and module 3 was mainly associated with Hippo signaling pathway. Conclusion Taken above, using integrated bioinformatical analysis, we have identified DEGs candidate genes and pathways in role of astrocyte involved in glaucoma with ocular hypertension, which could improve our understanding of the cause and underlying molecular events, and these candidate genes and pathways could be therapeutic targets for glaucoma.关键词
青光眼/眼高压/反应性视神经乳头星形胶质细胞/核心基因/通路富集Key words
Glaucoma/Ocular hypertension/Reactive optic nerve head astrocytes/Hub gene/Pathway enrichment引用本文复制引用
杨毅敬,项宇,田野,夏飞,周亚沙,彭俊,彭清华..基于生物信息学整合分析探讨青光眼高压反应性视神经星形胶质细胞相关核心基因[J].数字中医药（英文）,2018,1(4):280-288,9.基金项目
We thank for the finding support from the China National Natural Science Foundation Funding Project(NO. 81804150) （NO. 81804150）
Hunan University of Chinese Medicine, National Key Discipline of TCM Diagnostics Foundation Funding Project (No. 2015ZYZD02) （No. 2015ZYZD02）
The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine （）
Hunan Provincial Department of Education Innovation Platform Open Fund Project (16K065) （16K065）
Chinese Medicine Key Laboratory of Prevention and Treatment of Disease in Hunan Province (2017TP1018) （2017TP1018）
Changsha Science and Technology Plan Project(KC1704005) （KC1704005）
Hunan Engineering Technology Research Center for the Prevention and Treatment of Otorhinolaryngologic Diseases and Protection of Visual Function with Chinese Medicine （）
Hunan Provincial Research Innovation Project for Graduate students(CX2017B426). （CX2017B426）
