数字中医药（英文）2018，Vol.1Issue(4)：302-309,8.
水线草质量控制体系构建
Establishing a Quality Control System of Hedyotis corymbosa
摘要
Abstract
Objective Hedyotis corymbosa ( Shui Xian Cao, 水线草, HC) is the main antitumor medicine in China and other southeast Asian countries. A comprehensive quality control system of HC was established in this study, as the current system was ineffective. Methods Microscopy was used to observe the characteristics of the powder and cross-sectional tissue. Thin layer chromatography (TLC) was employed to separate HC from Hedyotis diffusa (Bai Hua She She Cao, 白花蛇舌草, HD). Simultaneously, high-performance liquid chromatography (HPLC) was used to establish the characteristic spectrum of HC and determine the concentrations of hedyotiscone A. Regarding the issue of systematicness, concentrations of water, total ash, acid-insoluble ash, heavy metals (including lead, arsenic, mercury, cadmium and copper) and pesticide residue were measured by the methods described in the 2015 edition of Chinese Pharmacopoeia. Results Calcium oxalate needle crystal bundles and oil drops were observed in the cross-sectional tissue. Three characteristic blue fluorescent spots were observed in the HC samples by TLC. There were five distinct characteristic peaks in HC but not in HD, and hedyotiscone A was the sharpest characteristic peak. The generated linear equation of hedyotiscone A was Y = 3.15 × 108X + 312.04 (r2 = 1.000) between 0.00005270 and 0.002108 mg/mL, and the average recovery was 100.42％. Precision [relative standard deviation (RSD) 0.92％], repeatability (RSD 0.14％), and recovery (RSD 0.85％) met the requirements for quantitative analysis. Hedyotiscone A concentrations ranged from 0.00017％～0.00113％ across different habitats. Concentrations of water, ash and acid-insoluble ash were 8.80％～11.2％, 7.00％～15.3％ and 0.440％～7.70％, respectively. Concentrations of Pb, As, Hg, Cd and Cu were 1.60～4.60, 0.650～1.60, 0.0300～0.100, 0.240～0.690 and 10.1～16.6 μg/g, respectively. Pesticide residues were less than 0.00100 ppm. Conclusion The quality control system can effectively distinguish HC from HD, so as to comprehensively control the quality of HC, to provide references for the clinical use of HC.关键词
水线草/白花蛇舌草/耳草酮A/薄层法/高效液相色谱法(HPLC)/质量标准Key words
Hedyotis corymbosa/Hedyotis diffusa/Hedyotiscone A/TLC/HPLC/Quality standard引用本文复制引用
林艳,李洪权,夏伯候,张智敏,李亚梅,张晓青,吴萍,林丽美,廖端芳..水线草质量控制体系构建[J].数字中医药（英文）,2018,1(4):302-309,8.基金项目
We thank for the funding support from the National Science foundation of China (No. 81503041), the Science ResearchProjects ofChinese Pharmacopoeia (No. Z18), the Science Research Projects of the Hunan Provincial Department of Education (No. 17C1213), the Science Research Projects of the Hunan Provincial Department of Education (No. 14C0860), and the Key Projects of the Changsha Municipal Science and Technology Bureau (No. K1406030-31). （No. 81503041）
