中国卒中杂志2019，Vol.14Issue(9)：865-871,7.DOI:10.3969/j.issn.1673-5765.2019.09.003
活化蛋白C通过降低炎症因子表达减轻大鼠缺血性脑损伤研究
Activated Protein C Alleviate Ischemic Brain Injury by Anti-inflammation
摘要
Abstract
Objective To explore whether activated protein C (APC) plays a protective role in ischemic brain injury. Methods The models of cerebral ischemia-reperfusion were performed by clipping right middle cerebral artery (MCA) of male SD rats and releasing MCA after 2 hours of ischemia. The rats were randomly divided into three groups: sham operation group, normal saline (NS) control group and APC group, with 6-8 rats in each group. After 6 hours of ischemia, the rats in sham operation group and control group were given NS (1 mL/kg) by intraperitoneal injection, and the rats in APC group were given APC (2 mg/kg). At 24 hours after clipping MCA, the neurological function scores, infarct volume and the permeability of blood-brain barrier (BBB), the expression of protein NF-κB p65 and IκB and inflammatory factors TNF-α and IL-1β in brain tissue were measured to analyze the effect of APC in cerebral ischemia. Results Compared to control group, the neurological function score significantly improved, the infarct volume and BBB permeability both reduced in APC group. Compared to control group, the expression of proteins NF-κB p65 and IκB in the cytoplasm increased, the expression of protein NF-κB p65 in cell nuclei decreased, and the levels of TNF-α and IL-1β in the cytoplasm significantly decreased in APC group. Conclusions APC can reduce the levels of inflammatory factors TNF-α and IL-1β, reduce BBB permeability by inhibiting the activation and nuclear translocation of NF-κB p65. APC plays a neuroprotective role in cerebral ischemia-reperfusion injure.关键词
活化蛋白C/脑缺血/炎症因子Key words
[Key Words] Activated protein C/ Cerebral ischemia/ Inflammatory factor引用本文复制引用
王金桥,杨晓晓,饶高峰..活化蛋白C通过降低炎症因子表达减轻大鼠缺血性脑损伤研究[J].中国卒中杂志,2019,14(9):865-871,7.基金项目
浙江省医药卫生科技计划项目(2018KY918) （2018KY918）
