肿瘤药学2019，Vol.9Issue(5)：726-730,5.DOI:10.3969/j.issn.2095-1264.2019.05.05
蟛蜞菊内酯对人肾癌细胞的体外生长抑制作用
Inhibition of Wedelolactone on the Growth of Human Renal Cancer Cells in Vitro
仙淑丽 1罗清琼2
作者信息
- 1. 上海交通大学医学院附属第九人民医院肾脏内科,上海,200011
- 2. 上海交通大学医学院附属第九人民医院检验科,上海,200011
- 折叠
摘要
Abstract
Objective To investigate the inhibitory effect of wedelolactone on human renal cancer cells and its related mechanisms. Methods Methythiazolyltetrazolium (MTT) was used to observe the effect of wedelolactone on the viability of renal cancer cells. Flow cytometry was used to detect the effects of wedelolactone on apoptosis and the cell cycle of renal cancer cells. Changes in apoptosis and expression of cycle-associated proteins were examined by Western blotting. Results Wedelolactone could inhibit the growth of renal can-cer cells ACHN and 786-O in a dose-dependent manner. It could significantly induce apoptosis in renal cancer cells and block the cell cycle in G0/G1 phase. Wedelolactone treatment could significantly up-regulate the expression of the pro-apoptotic protein Bax and inhibit the expression of anti-apoptotic proteins Bcl-2 and Bcl-xL. In addition, the wedelolactone could also inhibit the expression of the cell cycle-associated proteins cyclin D1, CDK4 and CDK6. Conclusion Wedelolactone could inhibit the growth of renal cancer cells in vitro, and it could be used as a potential drug for the treatment of renal cell carcinoma.关键词
蟛蜞菊内酯/肾癌/细胞凋亡/周期阻滞Key words
Wedelolactone/Renal cell carcinoma/Apoptosis/Cycle arrest分类
医药卫生引用本文复制引用
仙淑丽,罗清琼..蟛蜞菊内酯对人肾癌细胞的体外生长抑制作用[J].肿瘤药学,2019,9(5):726-730,5.
