临床神经外科杂志2019，Vol.16Issue(6)：472-475,4.DOI:10.3969/j.issn.1672-7770.2019.06.003
TSA联合HSV-Ⅰ型对C6细胞增殖、凋亡的影响
Effects of TSA combined with HSV-1 on proliferation,apoptosis in C6 cells
摘要
Abstract
Objective C6 cells are cultured in vitro as models to observe kojic ancient bacteriostatic A ( TSA ) combine type Ⅰ herpes simplex virus ( HSV-1 ) effects on tumor cell proliferation and apoptosis.Methods C6 cells in vitro were treated separately with 0.5 ×10 -3 μmol／L TSA, 10MOI HSV-1 and 0.5×10 -3 μmol／L TSA combined with 10MOI HSV-1 for 48 h and 72 h.The CCK-8 assay was used to observe the proliferation of C 6 cells.The rate of apoptosis was detected by Flow Cytometry.Results The proliferation activity rate of TSA +hsv-1 group was lower than that of TSA group and HSV-1 group 48 h later( P＜0.05, P＜0.01).After 72 h, the proliferation activity rate of C6 cells in the TSA +HSV-1 combined group was significantly lower than that in the TSA group and the HSV-1 group(all P＜0.01).At 48 h and 72 h, the apoptosis rate of C6 cells in TSA+HSV-1 group was significantly higher than that in TSA group and HSV group( all P＜0.01).Conclusion TSA combined with HSV-1 has synergistic or superimposed killing effect on C6 cells cultured in vitro.关键词
曲古抑菌素A/单纯疱疹病毒Ⅰ型/胶质瘤/增殖/凋亡Key words
TSA/type Ⅰherpes simplex virus/glioma/proliferation/apoptosis分类
生物科学引用本文复制引用
许强华,刘甜,陈新军,陈治军,刘平非,谢腾,陈晓巍..TSA联合HSV-Ⅰ型对C6细胞增殖、凋亡的影响[J].临床神经外科杂志,2019,16(6):472-475,4.基金项目
荆门市引导性科技项目(YDKY2015007) （YDKY2015007）
