临床神经外科杂志2019，Vol.16Issue(6)：476-480,5.DOI:10.3969/j.issn.1672-7770.2019.06.004
胶质瘤干细胞的结肠癌转移相关基因1表达与替莫唑胺耐药的相关性
Correlation between the expression of MACC1 and drug resistance of temozolomide in glioma stem cells
摘要
Abstract
Objective To investigate the relationship between metastasis-associated in colon cancer 1 ( MACC1 ) gene expression and the resistance of glioma stem cell to temozolomide ( TMZ).Methods The expression of MACC1 in U87 glioma cell-derived stem cells ( GSCs-U87) , U251 glioma cell-derived stem cells( GSCs-U251 ) , U87 and U251 was determined by quantitative real-time PCR and Western blotting.The silencing effect of RNA interference on MACC1 was detected by quantitative real-time fluorescence PCR and Western blot.The effect of MACC1 silencing on TMZ-induced cytotoxicity and apoptosis was measured by flow cytometry and CCK-8.Results The expression of MACC1 in GSCs-U87 and GSCs-U251 in glioma stem cells was up-regulated remarkably at mRNA and protein level( all P＜0.05 ).shRNA-MACC1 significantly inhibited MACC1 gene expression ( all P ＜0.05 ).The cytotoxicity of TMZ at various concentrations significantly increased in MACC1-silenced GSCs-U87 and GSCs-U251 cells compared with the control group GSCs-U87 and GSCs-U251.In addition, the apoptosis rate of MACC1-silenced GSCs-U87 and GSCs-U251 induced by TMZ significantly increased compared with the control group GSCs-U87 and GSCs-U251.Conclusions There is a relationship between overexpression of MACC1 and the characteristics of glioma stem cells.The silencing of the MACC1 gene enhances the effect of TMZ on the apoptosis and growth of glioma stem cells , thereby increasing the sensitivity of glioma stem cells to TMZ chemotherapy.关键词
胶质细胞瘤/MACC1/替莫唑胺/药物抵抗Key words
glioma/MACC1/temozolomide/drug resistance分类
生物科学引用本文复制引用
吕华荣,胡胜,吴星,荣亮,申松波,方媛..胶质瘤干细胞的结肠癌转移相关基因1表达与替莫唑胺耐药的相关性[J].临床神经外科杂志,2019,16(6):476-480,5.基金项目
湖北省自然科学基金项目(2014CFB421) （2014CFB421）
湖北省黄石市医疗卫生科技攻关计划项目[(2010)1号] （2010）
