临床神经外科杂志2019，Vol.16Issue(6)：481-486,6.DOI:10.3969/j.issn.1672-7770.2019.06.005
Eya3通过Hippo信号通路调控胶质瘤细胞化疗耐药的研究
Studies on role of Eya3 on chemoresistance of glioma cells by regulating Hippo signaling pathway
摘要
Abstract
Objective To explore whether Eya3 protein ( Eyes absent homolog 3) affects the chemoresistance of glioma cells by regulating the Hippo pathway.Methods Immunohistochemistry and Western blotting were used to detect the expression of Eya 3 in glioma tissues and cell lines.The effect of overexpression or silencing of Eya 3 on chemotherapy resistance of U 87 cells was detected by MTT assay.The effects of over-expression or silencing of Eya3 on the stemness and migration／invasion ability of U87 cells were examined by three-dimensional balling and migration／invasion experiments, respectively.The effect of Eya3 on the phosphorylation of Hippo pathway was detected by Western blotting.The transcription levels of Sox 2 and Slug genes were detected by ChIP (chromatin immunoprecipitation assay ) assay.Results Eya3 was highly expressed in glioma tissues and cells.After overexpression of Eya3, the resistance , stemness, migration and invasion ability of U87 cells increased significantly , while silencing of Eya3 was the opposite.Eya3 inhibits the phosphorylation of Hippo pathway and promotes the nuclear translocation of YAP .The increased YAP enhances the transcription of Sox 2 and Slug genes.Conclusion Eya3 affects chemoresistance of glioma cells by regulating the activity of Hippo pathway.关键词
眼缺失同源物3/干性维持/替莫唑胺耐药/Hippo通路/胶质瘤Key words
Eya3/stemness/temozolomide-resistance/Hippo pathway/glioblastoma分类
医药卫生引用本文复制引用
周培杰,钱中润,刘伟,罗文广,张红雁..Eya3通过Hippo信号通路调控胶质瘤细胞化疗耐药的研究[J].临床神经外科杂志,2019,16(6):481-486,6.基金项目
安徽省自然科学基金(1908085QH338 （）
1808085MH266) （）
