广西医科大学学报2019，Vol.36Issue(11)：1705-1712,8.DOI:10.16190/j.cnki.45-1211/r.2019.11.001
RSK4剪接变异体2对人乳腺癌细胞MDA-MB-231增殖和侵袭的影响
Effect of RSK4 splice mutant 2 on proliferation and invasion of human breast cancer MDA-MB-231 cells
摘要
Abstract
Objective:To investigate the effects of human ribosomal S6 protein kinase 4 mutant 2 (RSK4m2)on proliferation,migration and invasion of breast cancer MDA-MB-231 cells.Methods:MDA-MB-231 cells were transfected with lentivirus vector carrying RSK4m2 (RSK42)to specifically up-regulate the expression of RSK4m2,or transfected with lentivirus negative empty vector as a negative control group.And the untransfected MDA-MB-231 cells were set as a blank control group.The transfection efficiency was confirmed by PCR and western blotting.The proliferation,invasion,and migration of MDA-MB-231 cells were respectively detected by CCK 8,colony formation assay,scratch assay and Transwell assay after transfection.Results:The mRNA and protein expression levels of RSK4rn2 were significantly increased in MDA-MB-231 cells after transfected with RSK4m2.The proliferation,migration and invasion abilities of RSK4m2 group were significantly decreased compared with other two groups(P＜0.05).There was no significant difference between the negative control and blank control groups (P＞0.05).Conclusion:RSK4m2 is involved in the regulation of biological behavior of human breast cancer MDA-MB-231 cells in vitro,and it can inhibit cell proliferation,migration and invasion.关键词
剪接变异体/乳腺癌/RSK4m2/细胞增殖/迁移/侵袭Key words
splice mutant/breast cancer/ribosomal protein S6 kinase 4 mutant 2/cell proliferation/cell migration/cell invasion分类
医药卫生引用本文复制引用
梅燕,朱玲钰,杨泓熇,钟国斌,杨华伟..RSK4剪接变异体2对人乳腺癌细胞MDA-MB-231增殖和侵袭的影响[J].广西医科大学学报,2019,36(11):1705-1712,8.基金项目
This work was supported by the National Natural Science Foundation of China (No.81860464 （）
No.8186100068) （）
