数字中医药（英文）2019，Vol.2Issue(1)：41-49,9.DOI:10.1016/j.dcmed.2019.05.005
α-葡萄糖苷酶抑制剂活性指导下的HPLC-Q-TOF-MS/MS鉴定臭牡丹中化学成分
α-Glucosidase Inhibitory Activity-guided Identification of Compounds from Clerodendrum bungei Steud by HPLC-ESI-QTOF-MS/MS
摘要
Abstract
Objective To identify the compounds with α-glucosidase inhibitory activity from Clerodendrum bungei Steud (Chou Mu Dan,臭牡丹） using HPLC-ESI-QTOF-MS/MS.Methods The ethanol extracts of Clerodendrum bungei Steud (Chou Mu Dan,臭牡丹) were partitioned with petroleum ether,ethyl acetate,n-butanol,and water.The assay for α-glucosidase inhibitory activity revealed strong α-glucosidase inhibitory activity in the ethyl acetate fraction,and the bioactive compounds present in this fraction were identified by the HPLC-ESI-QTOF-MS/MS method.Results A total of 29 compounds were determined,among the identified bioactive components;these included 12 phenylethanoid glycosides (compounds 5,6,12-17,20-22,24),7 flavonoids (compounds 10,19,23,25-28),5 phenolic acids (compounds 2-4,7,9),and 5 other compounds.Compounds 2-4,7,9-10,12-13,15,19,and 26,with a potential α-glucosidase inhibitory activity,have been reported previously.Conclusions Our results show that the methodology used in this study is feasible,credible,and rapid in identifying known compounds and also for characterizing new natural α-glucosidase inhibitory candidates from Clerodendrum bungei Steud (Chou Mu Dan,臭牡丹).关键词
α-葡萄糖苷酶抑制剂/臭牡丹/高效液相-四级杆-飞行时间-质谱Key words
α-glucosidase inhibitor/Clerodendrum bungei Steud (Chou Mu Dan,臭牡丹)/HPLC-ESI-QTOF-MS/MS引用本文复制引用
黄小龙,万丹,舒骏,方良子,邹笃准,张水寒..α-葡萄糖苷酶抑制剂活性指导下的HPLC-Q-TOF-MS/MS鉴定臭牡丹中化学成分[J].数字中医药（英文）,2019,2(1):41-49,9.基金项目
We would like to thank Ms.YU Na from Hunan University of Chinese Medicine for the help in the collection of reference plant.We also thank for the funding support from the China National Natural Science Foundation Funding Project (NO.81503452),Natural Science Foundation of Hunan Province,China (No.14JJ4066). （NO.81503452）
