数字中医药（英文）2019，Vol.2Issue(1)：50-58,9.DOI:10.1016/j.dcmed.2019.05.006
基于UPLC-QTOF/MS和UNIFI二元比较鉴别巴西龟龟甲胶和草龟龟甲胶
Differentiating Trachemys scripta elegans Shell Glue from Chinemys reevesii Shell Glue by UPLC-QTOF/MS Coupled with Binary Compare Tool of UNIFI
摘要
Abstract
Objective This study was conducted to develop an method for identification of Trachemys scripta elegans (Ba Xi Gui,巴西龟)shell glue from Chinemys reevesii (Zhong Hua Cao Gui,中华草龟)shell glue.Methods In this research,an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) method coupled with Binary Compare tool of UNIFI software was validated to differentiate Trachemys scripta elegans (Ba Xi Gui,巴西龟)shell glue from Chinemys reevesii (Zhong Hua Cao Gui,中华草龟)shell glue.The gelatins were digested enzymatically into peptides using trypsin.The UPLC-QTOF/MS data of the trypsin digested samples was subjected to Binary Compare of UNIFI software in order to screen out the unique peptides.PEAKS(R) Studio software was utilized to identify the sequence of the marker peptides.Results It was found that m/z 641.3 was the marker peptide in samples of Trachemys scripta elegans (Ba Xi Gui,巴西龟)shell glue,and m/z 649.3 was the marker peptide in samples of Chinemys reevesii (Zhong Hua Cao Gui,中华草龟)shell glue,with possible amino acid sequence of GEAGPSGPAGPTGAR and GESGPSGPAGPTGAR respectively.Conclusions The results will be helpful for the differentiating between these 2 gelatins,and it can be also used for quality control of Tortoise shell glue (Gui Jia Jiao,龟甲胶).关键词
超高效液相色谱-四极杆飞行时间质谱/龟甲胶/巴西龟/中华草龟Key words
UPLC-QTOF/MS/Tortoise shell glue/Trachemys scripta elegans (Ba Xi Gui,巴西龟)/Chinemys reevesii (Zhong Hua Cao Gui,中华草龟)引用本文复制引用
唐敏,严建业,任杰,李顺祥..基于UPLC-QTOF/MS和UNIFI二元比较鉴别巴西龟龟甲胶和草龟龟甲胶[J].数字中医药（英文）,2019,2(1):50-58,9.基金项目
We thank for the funding support from the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province ([2010]212),Changsha City Science and Technology Project in 2015 (k1501024-31),Key Discipline Program of State Administration of Traditional Chinese Medicine of the P.R.C ([2009]30),Key Discipline in Hunan Province ([2011]76),Provincial Natural Science Foundation of Hunan (2017JJ3234),Key Project of Science and Technology of Hunan Province (2016SK2033). （[2010]212）
