数字中医药（英文）2019，Vol.2Issue(2)：72-85,14.DOI:10.1016/j.dcmed.2019.09.002
痰湿证肥胖大鼠肾周脂肪基因甲基化特征及温胆汤干预的影响
Methylation Characteristics of Perivisceral Fat Gene in Obese Rats with Phlegm-dampness Syndrome and the Effect of Wen Dan Decoction
摘要
Abstract
Objective To observe the characteristics of gene methylation in obese rats with phlegm-dampness syndrome induced by the high-fat diet, and to study the effect of Wen Dan Decoction on gene methylation after the intervention. Methods Methylation sites of genes were detected by the MeDIP-seq method. Bioinformatics method was used to analyze the gene methylation characteristics of obesity with phlegm-dampness syndrome and the effect of Wen Dan Decoction. Results (1) There were 3242 methylation differential loci in diet-induced obesity with phlegm-dampness syndrome, of which 1243 were down-regulated and 1999 were up-regulated, involving 1579 differential genes. GO analysis showed that "offactory receptor activity" and others were enriched. The possible signal pathways involved were "Olfactory transduction" "Tuberculosis""Systemic lupus erythematosus" and "Ribosome". (2) After the intervention of Wen Dan Decoction in obesity with phlegm-dampness syndrome, 4046 different methylation loci were obtained, including 1067 down-regulated loci and 2979 up-regulated loci, involving 2068 genes. GO analysis showed that "offactory receptor activity" and others were enriched. These genes involved seven signaling pathways, such as "Metabolic pathways". (3) Between diet-induced obesity with phlegm-dampness syndrome and Wen Dan Decoction intervening obesity with the phlegm-dampness syndrome, 582 common genes of methylation differential genes were obtained. After the intervention of Wen Dan Decoction, the number of GO enrichment items was more than that of obesity with phlegm-dampness syndrome, and even the same GO enrichment items involved more genes. Conclusions The phlegm-dampness syndrome of obesity-induced by diet had the characteristics of gene methylation changes, and the intervention of Wen Dan Decoction could also affect the status of gene methylation. The genes affected by Wen Dan Decoction were closely related to the methylation gene of phlegm-dampness syndrome of obesity-induced by diet but covered a wider range.关键词
肥胖/痰湿证/基因甲基化/温胆汤/生物信息学Key words
Obesity/Phlegm-dampness syndrome/Gene methylation/Wen Dan Decoction/Bioinformatics引用本文复制引用
杨海燕,喻松仁,夏循礼,程绍民,王萍..痰湿证肥胖大鼠肾周脂肪基因甲基化特征及温胆汤干预的影响[J].数字中医药（英文）,2019,2(2):72-85,14.基金项目
We thank for the funding support from the National Natural Science Youth Foundation of China: Effect of Wen Dan Decoction on gene promoter methylations related to fat metabolism (No. 81302907). （No. 81302907）
