数字中医药（英文）2019，Vol.2Issue(2)：97-104,8.DOI:10.1016/j.dcmed.2019.09.004
密蒙花颗粒对去势雄兔泪腺细胞炎症因子TGF-β1,NF-κB,IL-10和IL-12表达的影响
Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1, NF-κB, IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits
摘要
Abstract
Objective To observe the effects of Buddlejae Flos (Mi Meng Hua,密蒙花) granules on the expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland cells in castrated male rabbits. Methods A total of 30 New Zealand male white rabbits were randomly divided into 5 groups. Group A: blank group; group B:model group; group C: Buddlejae Flos (Mi Meng Hua,密蒙花) granules group; group D: placebo group; group E: testosterone group. Except for the group A, all rabbits were treated with bilateral testicular and epididymis resection before intervention. Rabbits in group C were administered with Buddlejae Flos (Mi Meng Hua,密蒙花) granules (100 mg/kg), 3 times per day;Rabbits in group D were administered with normal saline, 3 times per day. Rabbits in group E were injected with testosterone propionate (0.5 mL/kg) in the thigh muscle, every 3 days. Schirmer I test (SIT) and break-up time (BUT) were measured on 1st day before modeling and 30th day after modeling. After 30 days of intervention, all rabbits were sacrificed. The removal lacrimal gland was stained in immunohistochemica staining. The expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland tissue of each group was detected, and the structure of lacrimal gland was observed. Results The results of SIT and BUT showed that there was significant difference between group B and D compared with group A (P < 0.05). There were no significant differences between group C and E (P > 0.05). There were no significant differences in the comparison of inflammatory factors TGF-β1, NF-κB, IL-10 and IL-12 between group B and D (P > 0.05). There were significant differences between group B and D compared withother groups (P < 0.01). There was no significant difference between group A, C and E (P > 0.05). Conclusions Buddlejae Flos (Mi Meng Hua,密蒙花) granules have an inhibitory effect on the expression of TGF-β1, NF-κB, IL-10 and IL-12 in castrated male rabbit lacrimal gland cells, and alleviate the inflammation of lacrimal gland tissue by inhibiting the expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland tissue, so as to treat dry eye.关键词
密蒙花颗粒/去势雄兔/泪腺组织/炎症因子/干眼Key words
Buddlejae Flos (Mi Meng Hua 密蒙花) granules/Castrated male rabbit/Lacrimal gland tissue/Inflammatory factors/Dry eye引用本文复制引用
蒋鹏飞,彭俊,谭涵宇,欧阳云,覃艮艳,彭清华..密蒙花颗粒对去势雄兔泪腺细胞炎症因子TGF-β1,NF-κB,IL-10和IL-12表达的影响[J].数字中医药（英文）,2019,2(2):97-104,8.基金项目
We thank for the funding support from the National Natural Science Foundation of China (No. 30772824 and No. 81574031), Science and Technology Plan of Changsha City (No. K1501014-31 and No. kc1704005), Central Government Supports the Construction of Local Colleges and Universities Project, Key Subject Construction Project of Traditional Chinese Medicine Ophthalmology of the National Administration of Traditional Chinese Medicine and Construction Project of Key Discipline of Traditional Chinese Medicine in Hunan Province. （No. 30772824 and No. 81574031）
