数字中医药（英文）2019，Vol.2Issue(2)：117-124,8.DOI:10.1016/j.dcmed.2019.09.006
土家药白三七三萜皂甙及其对Hep G2和BGC-823细胞系的细胞毒性
Triterpenoid Saponins from Tu Jia Ethnomedicine Bai San Qi and Their Cytotoxicity on Hep G2 and BGC-823 Cell Lines
摘要
Abstract
Objective Tu Jia ethnomedicine is a unique medical system inherited and adhibited by Tu Jia minority living in central-south China.Panax japonicus C. A. Mey. (Bai San Qi,白三七) is recognized as an effective and rare medicinal plant to treat weakness, fatigue and rheumatism in Tu Jia ethnomedicine. This paper is to discover more substance evidence for the application of Tu Jia ethnomedicine. Methods Column chromatography and preparative high performance liquid chromatography (HPLC) was applied for isolation and purification;1H-NMR,13C-NMR,1H-1H COSY, HSQC and HMBC NMR spectra were applied for structure identification; Methyl thiazolyl tetrazolim (MTT) assays were applied for cytotoxicity evaluation. Results Totally 12 known compounds were isolated by column chromatography and preparative HPLC from rhizomes ofPanax japonicus C. A. Mey. (Bai San Qi,白三七). Structures of these compounds were identified by their NMR spectra. All the 12 compounds were triterpenoid saponins. Five of them were oleanolic acid type, and the remaining 7 were dammarane type. Eleven compounds were assayed for their cytotoxic activity against Hep G2 human liver cancer cell lines and BGC-823 human gastric cancer cell lines. Three of the 11 showed relatively dominant cytotoxicity against these cell lines. Conclusions A total of 12 known compounds have been identified fromPanax japonicus C. A. Mey. (Bai San Qi,白三七);NMR spectra of compounds with similar skeletons showed regular characteristics; 3 compounds showed relatively dominant cytotoxicity against Hep G2 and BGC-823 cancer cell lines, and the result can be valued as weak while setting the taxol as a positive control.关键词
三萜/皂苷/细胞毒性/五加科/白三七/土家族民族药Key words
Triterpenoid/Saponin/Cytotoxicity/Araliaceae/Panax japonicus C. A. Mey. (Bai San Qi 白三七)/Tu Jia ethnomedicine引用本文复制引用
李伟,Muhammad Daniyal,谢青玲,刘杨,谢谦,李斌,彭彩云,王炜..土家药白三七三萜皂甙及其对Hep G2和BGC-823细胞系的细胞毒性[J].数字中医药（英文）,2019,2(2):117-124,8.基金项目
We thank for the funding support from the National Natural Science Foundation of China (No. 81703819 and No. 81874369), Hunan Key Laboratory of Druggability and Preparation Modification for Traditional Chinese Medicine (No. 2017-04), Hunan Provincial Key Laboratory of Dong Medicine (No. 2015TP1020-02) and Students Research Innovative Program of Hunan Province (No. 2018-413). （No. 81703819 and No. 81874369）
