烟草科技2019，Vol.52Issue(11)：1-9,9.
高脲酶活性褐土的宏基因组测序与微生物来源脲酶基因的筛选
Metagenomic sequencing analysis of cinnamon soil with high urease activity and identification of urease genes from microbe
摘要
Abstract
To investigate the source of urease-producing microbes in cinnamon soil, the urease activity of cinnamon soil samples collected from Zhengzhou, Luoyang, Xuchang, Pingdingshan and Anyang in Henan Province were measured. Five samples with the highest urease activity in each city were cultured in liquid medium containing high concentration urea to enrich urease-producing microbes. The DNA in a mixture of the five samples was extracted and used to construct a metagenomic library, and the urease-producing microbes in cinnamon soil were analyzed with metagenome sequencing technology. The results showed that the urease activity of cinnamon soil ranged from 8.34 to 67.18 μ g·d-·1g-1, and the highest urease activities were 46.98, 67.18, 57.68, 45.97 and 64.15 μ g·d-·1g-1 at the five sampling points. Via sequence assembly with metagenome sequencing data, 406 urease structural proteins and 437 urease accessory proteins were identified. Species annotation indicated that the urease-producing microbes in cinnamon soil were mainly bacteria, plus a few types of fungi and archaeas. The urease-producing bacteria closely related to Streptomyces, Arthrobacter and Acinetobacter. The urease-producing fungi had close relationships with Madurella mycetomatis, Neofusicoccum parvum and Chaetomium thermophilum. The urease-producing archaeas were closely related to Nitrososphaera and Nitrosopumilus, which suggested that archaeas might be involved in urea degradation in cinnamon soil as well.关键词
褐土/脲酶活性/宏基因组学/脲酶/脲酶辅助蛋白/微生物Key words
Cinnamon soil/Urease activity/Metagenomics/Urease/Urease accessory protein/Microbe分类
轻工纺织引用本文复制引用
轩贝贝,周汉平,张艳玲,胡利伟,宋纪真,郭建华,王爱国,牟文君,戴华鑫,闫鼎,蔡宪杰,奚家勤,薛超群..高脲酶活性褐土的宏基因组测序与微生物来源脲酶基因的筛选[J].烟草科技,2019,52(11):1-9,9.基金项目
河南省自然科学基金资助项目"基于宏基因组和宏转录组学研究褐土脲酶群落构成与抑制效应分析"(122018AS0020) （122018AS0020）
河南省自然科学基金资助项目"小麦-土壤系统中邻苯二甲酸酯的污染特征及植株响应分子机制研究"(112018AS0030) （112018AS0030）
国家自然科学基金资助-青年科学基金项目"青枯病菌对氟啶胺的抗性机制研究"(122018AZ0330). （122018AZ0330）
