烟草科技2019，Vol.52Issue(11)：10-16,7.
休耕对连作障碍植烟土壤酶活性及细菌群落结构的影响
Effects of lying fallow on enzyme activities and bacterial community structures in tobacco-planting soils with continuous cropping obstacles
摘要
Abstract
To investigate possible mechanisms of lying fallow on the microecological environment of soils with continuous cropping obstacles, the effects of lying fallow on the enzyme activities and bacterial community structure in soils was studied in continuous tobacco cropping fields with severe bacterial wilt. The results showed that lying fallow effectively promoted the contents of organic matter, alkali-hydrolyzed nitrogen and enzyme activities in soils. Especially after two fallow years, soil invertase and catalase activities increased by 147.85% and 64.13% respectively, as compared with continuous tobacco cropping. Lying fallow was beneficial to promote the abundance of bacterial community in soils. With the increase of fallow years, Chao index and Shannon index increased, while Simpson index decreased. The relative abundances of beneficial bacteria Acidobacteria and Roseiflexus were significantly higher, while that of pathogenic bacteria Ralstonia was significantly lower in the fields after lying fallow than in continuous tobacco cropping fields. The incidence of bacterial wilt in tobacco fields lying fallow for one or two years was lower by 22.62% or 32.55% than that in continuous tobacco cropping fields, respectively. Therefore, lying fallow improves the microecological environment and fertility of tobacco-growing soils with continuous cropping obstacles and reduces the incidence of bacterial wilt.关键词
植烟土壤/休耕/连作障碍/青枯病/微生态/酶活性/细菌群落结构Key words
Tobacco-planting soil/Lying fallow/Continuous cropping obstacle/Bacterial wilt/Microecology/Enzyme activity/Bacterial community structure分类
农业科技引用本文复制引用
左梅,谭军,向必坤,施河丽,陈红华,彭五星..休耕对连作障碍植烟土壤酶活性及细菌群落结构的影响[J].烟草科技,2019,52(11):10-16,7.基金项目
中国烟草总公司科技重点项目"基于宏基因组学的植烟土壤健康评价研究与应用"(110201402016) （110201402016）
湖北省烟草公司科技重点项目"市场需求下恩施州核心烟区烟叶质量定位及关键技术调控"(027Y2017-003). （027Y2017-003）
