林产化学与工业2019，Vol.39Issue(6)：59-67,9.DOI:10.3969/j.issn.0253-2417.2019.06.008
五味子果肉和种子精油的化学成分与生物活性对比研究
Comparative Study on Essential Oils of Schisandra chinensis Fruits Pulp and Seeds: Chemical Composition and Bioactivities
摘要
Abstract
In order to investigate the differences and similarities between the essential oils (EOs) obtained from Schisandra chinensis fruits pulp and seeds (PEO and SEO), the chemical composition, anti-tumor property, antimicrobial and antioxidant activity were examined. According to the results of GC-MS (gas chromatography-mass spectrometry, GC-MS) analyses, 55 and 45 compounds which represented 94. 04% and 93. 39% of the oils were identified, respectively. In addition, anti-tumor activity of essential oils was carried out using the Sulforhodamine B (SRB) method. The oils also revealed the highest anti-tumor activity against HeLa and BGC-823 cells, but they had no effect on A549 cells. They presented IC50 values from 39. 07 mg/ L to 56. 43 mg/ L. PEO and SEO also exhibited inhibitory activity against four bacterial strains including Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. MIC of PEO were 245. 00 - 504. 33 mg/ L, MIC of SEO were 495. 00 - 1 004. 68 mg/ L. Furthermore, in all assays of antioxidant ability, PEO showed stronger antioxidant properties than SEO. IC50 value of PEO against DPPH and ABTS radical were 3. 15 and 2.31 g/ L, respectively. IC50 of SEO against DPPH and ABTS radical were 5. 43 and 10. 11 g/ L, respectively. In a word, this study supports the potentials of PEO and SEO as antibacterial and antineoplastic agent.关键词
五味子/精油/抗肿瘤活性/抑菌活性/抗氧化活性Key words
Schisandra chinensis/essential oils/anti-tumor activity/antibacterial activity/antioxidant activity分类
化学化工引用本文复制引用
王洁,路娇娇,杜涵,张萌,刘盼盼,陈小强..五味子果肉和种子精油的化学成分与生物活性对比研究[J].林产化学与工业,2019,39(6):59-67,9.基金项目
中央高校基本科研业务费专项资金项目(2572015CB18) （2572015CB18）
东北林业大学大学生创新训练项目(201810225502) （201810225502）
黑龙江省自然科学基金项目(LH2019C006) （LH2019C006）
