首页|期刊导航|畜牧与生物技术杂志(英文版)|Influence of the concentration of dietarydigestible calcium on growth performance,bone mineralization, plasma calcium, andabundance of genes involved in intestinalabsorption of calcium in pigs
Influence of the concentration of dietarydigestible calcium on growth performance,bone mineralization, plasma calcium, andabundance of genes involved in intestinalabsorption of calcium in pigs
L. Vanessa Lagos Su A. Lee Guillermo Fondevila Carrie L. Walk Michael R. Murphy Juan J. Loor Hans H. Stein
畜牧与生物技术杂志(英文版)2019，Vol.10Issue(4)：942-957,16.
畜牧与生物技术杂志(英文版)2019，Vol.10Issue(4)：942-957,16.
Influence of the concentration of dietarydigestible calcium on growth performance,bone mineralization, plasma calcium, andabundance of genes involved in intestinalabsorption of calcium in pigs
摘要
Abstract
Background: A 21-day experiment was conducted to test the hypothesis that Ca requirements to maximize growth performance expressed as the standardized total tract digestible (STTD) Ca to STTD P ratio is less than 1.40: 1. The second hypothesis was that increasing dietary Ca increases plasma Ca concentration and downregulates abundance of genes related to Ca absorption (TRPV6, S100G, and ATP2B1) in the duodenum, and tight junction proteins (OCLN, CLDN1, and ZO1) in the duodenum and ileum. Methods: Twenty corn-soybean meal diets were formulated using a 4 × 5 factorial design with diets containing 0.16％, 0.33％, 0.42％, or 0.50％ STTD P, and 0.14％, 0.29％, 0.44％, 0.59％, or 0.74％ STTD Ca. Six hundred and forty pigs (initial weight: 11.1 ± 1.4 kg) were allotted to 20 diets and 5 blocks in a randomized complete block design. On day 21, weights of pigs and feed left in feeders were recorded and blood, duodenal tissue, ileal mucosa, and the right femur were collected from 1 pig per pen. Abundance of mRNA was determined in duodenal and ileal tissue via quantitative RT-PCR. Data were analyzed using a response surface model. Results: The predicted maximum ADG (614 g), G:F (0.65), and bone ash (11.68 g) was obtained at STTD Ca:STTD P ratios of 1.39:1, 1.25:1, and 1.66:1, respectively, when STTD P was provided at the requirement (0.33％). If dietary STTD P was below the requirement, increasing dietary Ca resulted in reduced (P < 0.05) ADG and G:F. However, if dietary STTD P was above the requirement, negative effects (P < 0.05) on ADG and G:F of increasing STTD Ca were observed only if dietary STTD Ca exceeded 0.6％. Plasma Ca concentration was positively affected by STTD Ca over the range studied (quadratic, P < 0.01) and negatively affected by increasing STTD P (linear, P < 0.01). There was a linear negative effect (P < 0.05) of STTD Ca on the abundance of S100G, TRPV6, OCLN, and ZO1 in duodenum, and CLDN and ZO1 in ileum.关键词
Bone ash/Calcium absorption/Digestible calcium/Growth/Pigs/RequirementKey words
Bone ash/Calcium absorption/Digestible calcium/Growth/Pigs/Requirement引用本文复制引用
L. Vanessa Lagos,Su A. Lee,Guillermo Fondevila,Carrie L. Walk,Michael R. Murphy,Juan J. Loor,Hans H. Stein..Influence of the concentration of dietarydigestible calcium on growth performance,bone mineralization, plasma calcium, andabundance of genes involved in intestinalabsorption of calcium in pigs [J].畜牧与生物技术杂志(英文版),2019,10(4):942-957,16.基金项目
Financial support for this research from AB Vista, Marlborough, UK. （）
