中国卒中杂志2019，Vol.14Issue(11)：1089-1094,6.DOI:10.3969/j.issn.1673-5765.2019.11.003
急性缺血性卒中患者血清miRNA-148a表达水平检测及其临床价值预探索
Clinical Value and Expression Level of Serum miRNA-148a in Patients with Acute Ischemic Stroke
郭桂珍 1韩荣胜 2王有清 1刘庆春1
作者信息
- 1. 1810000 西宁青海省第五人民医院神经内科
- 2. 青海省第五人民医院心内科
- 折叠
摘要
Abstract
Objective To investigate the level and clinical value of serum miRNA-148a in patients with acute ischemic stroke (AIS). Methods The serum samples of peripheral blood from 42 AIS patients and 42 healthy control participants were collected, and the miRNA-148a levels of all subjects were measured by a real-time fluorogenic quantitative PCR. The receiver operating characteristic (ROC) curve of miRNA-148a for the diagnosis of AIS was plotted, and the correlation between miRNA-148a expressioin level and clinical data were analyzed. The GSE55937 dataset were downloaded from Gene Expression Omnibus to measure the miRNA-148a expression level in AIS patients, and the target genes and biological functions of miRNA-148a were predicted using bioinformatics analysis. Results The miRNA-148a expression level in AIS patients within 12 h after onset was significantly lower than that in healthy control group (0.76±0.23 vs 1.02±0.21, P<0.001).The area under the ROC curve of miRNA-148a for the diagnosis of AIS was 0.79 (95% CI 0.70-0.89, P<0.001). Spearman correlation test showed that the miRNA-148a expression level in AIS patients were negatively associated with infarction size (r=-0.34, P=0.03) and high sensitivity C-reactive protein (hs-CRP) (r=-0.43, P=0.005). In GSE55937 dataset, the level of miRNA-148a was also remarkably lower in AIS than that in control group (1.93±0.46 vs 2.52±1.00, P=0.011), and the area under the ROC curve of miRNA-148a for the diagnosis of AIS was 0.69 (95% CI 0.54-0.84, P=0.022). A total of 58 target genes were predicted by bioinformatics methods, such as WNT10B and DNMT1. The molecular mechanism of the target genes mainly involve the regulation of cell apoptosis, FoxO signaling pathway and PI3K-Akt pathway and etc. Conclusions The serum miRNA-148a level is significantly decreased in the early phase of AIS patients, and the mechanism of miRNA-148a in stroke may involve cell apoptosis, FoxO signal pathway and PI3K-Akt signal pathway. miRNA-148a may have certain value in the diagnosis and assessment of AIS.关键词
缺血性卒中/miRNA-148a/标志物/生物信息学Key words
Ischemic stroke/miRNA-148a/Biomarker/Bioinformatics引用本文复制引用
郭桂珍,韩荣胜,王有清,刘庆春..急性缺血性卒中患者血清miRNA-148a表达水平检测及其临床价值预探索[J].中国卒中杂志,2019,14(11):1089-1094,6.
