动物营养学报2019，Vol.31Issue(11)：5081-5089,9.DOI:10.3969/j.issn.1006⁃267x.2019.11.023
多聚甲醛对牛瘤胃消化酶活性及营养物质消化的影响
Effects of Paraformaldehyde on Activities of Digestive Enzymes of Rumen and Nutrient Digestion in Cattle
摘要
Abstract
This experiment was aimed to investigate the effects of paraformaldehyde on activities of digestive enzymes of rumen and nutrient digestion in cattle. Four of Xinjiang brown×Simmental F1 non?pregnant cattle, 2?year?old, with body weight of (283.5±5.4) kg, were fitted with permanent ruminal fistula, and were fed a diet with a ratio of 4∶6 of concentrate to roughage ad libitum. By 4×4 Latin square design, each animal was supplemented with paraformaldehyde ( PFD ) at the additive amount of 0 ( control ) , 150, 300 and 600 mg／kg, respectively in each phase. The results showed that the dry matter voluntary intake of cattle was up?down changed with PFD supplementation, and was the highest at the PFD additive amount of 300 mg／kg, increasing by 25.6%( P＜0.01) compared with the control group. The activities of ruminal endocellulase, exo?cellulase, cellobiase and xylanase were up?down changed with the increasing of PFD additive amount, and were the highest at the PFD additive amount of 300 mg／kg, increasing by 38.1%( P＜0.01) , 37.0%( P＜0.01) , 45.9%( P＜0.01) and 25.0%( P＜0.05 ) , respectively. However, adding PFD had no significant effect on the activities of pectinase, amylase and protease ( P＞0.05). The apparent digestibility of dry matter, organic matter and cellulose, rather than crude protein, decreased significantly by PFD supplementation ( P＜0.05). However, the amounts of digested dry matter, organic matter, crude protein, cellulose and energy were up?down changed with PFD supplementation, being the highest at 300 mg／kg, by 18.5%( P＜0.01) , 18.7%( P＜0.01) , 20.4%( P＜0.01) , 17.9%( P＜0.01) and 14.6%( P＜0.01). The retention of nitrogen, calcium and phosphorus also were up?down changed with PFD supplementation, being the highest at 300 mg／kg, by 66.1%( P＜0.01) , 19.3%( P＜0.01) and 16.2%( P＜0.01), respectively. It is concluded that supplementation with PFD ( 300 mg／kg diet) increases the activities of ruminal cellulase, voluntary feed intake, the digested amounts of diets, and the nitrogen retention, so being beneficial for digestion and nutrition of cattle.[ Chinese Journal of Animal Nutrition, 2019, 31(11) :5080?5088]关键词
多聚甲醛/牛/瘤胃/消化Key words
paraformaldehyde/cattle/rumen/digestion分类
农业科技引用本文复制引用
雷智嘉,雒秋江,黄振,陈勇,臧长江..多聚甲醛对牛瘤胃消化酶活性及营养物质消化的影响[J].动物营养学报,2019,31(11):5081-5089,9.基金项目
国家自然科学基金项目(31772625) （31772625）
