动物营养学报2019，Vol.31Issue(11)：5143-5151,9.DOI:10.3969/j.issn.1006⁃267x.2019.11.029
短期冰水刺激对犬血常规、血清生化和免疫指标的影响
Effects of Short?Term Ice Water Stimulation on Blood Routine, Serum Biochemical and Immune Indexes of Canines
摘要
Abstract
The present study was to explore the effects of short?term ice water stimulation on blood routine, se?rum biochemical and immune indexes of canines. In this experiment, eight healthy Chinese rural dogs were se?lected and the blood samples were collected from the jugular vein for the determination of canine blood routine and serum biochemical and immune indexes before ice water stimulation, at the end of ice water stimulation ( after 0.5 h in ice water) and 0.5, 1.0, 3.0, and 7.0 h after the ice water stimulation. The results showed as follows: compared with before ice water stimulation, red blood cells count ( RBC) , hemoglobin concentration ( HGB) , hematocrit( HCT) , lymphocytes count ( Lym) , lymphocyte percentage ( Lym%) , monocyte per?centage ( Mon%) in blood and the activities of aspartate aminotransferase ( AST) , alanine aminotransferase ( ALT) and alkaline phosphatase ( ALP) and the contents of urea, uric acid ( UA) , creatinine, complement 3 ( C3) in serum were significantly increased at the end of ice water stimulation ( P＜0.05) ; at each recovery time point (0.5, 1.0, 3.0 and 7.0 h) , serum AST activities and urea content were significantly increased ( P＜0.05) ; at recovery time of 1.0, 3.0 and 7.0 h, blood HCT, RBC, HGB and Lym% were significantly de?creased ( P＜0.05) , while blood white blood cells count ( WBC) , granulocyte count ( Gran) and granulocyte percentage ( Gran%) were significantly increased ( P＜0.05 ) ; serum creatine kinase ( CK) activity and C3 content were significantly increased at recovery time of 0.5, 1.0 and 3.0 h ( P＜0.05) , however, serum creati?nine content and ALP activity were significantly decreased at recovery time of 1.0, 3.0 and 7.0 h ( P＜0.05). Compared with the end of ice water stimulation, blood RBC and Lym were significantly decreased at each re?covery time points ( P＜0.05) , and serum creatinine content and ALP activity were significantly decreased ( P＜0.05) ; however, blood HGB, HCT, Lym% and Mon% were decreased significantly at recovery time of 1.0, 3.0 and 7.0 h ( P＜0.05). The results indicate that short?term ice water stimulation can significantly change some blood routine, serum biochemical and immune indexes of canines, and suggest that the animals should in time to correct the changes of blood routine and serum biochemical and immune indexes, enhance the body im?munity, and alleviate the continuous harm when animals are stimulated by ice water.[ Chinese Journal of Ani?mal Nutrition, 2019, 31(11) :5142?5150]关键词
犬/短期冰水刺激/血常规指标/血清生化指标/血清免疫指标Key words
canine/short⁃term ice water stimulation/blood routine indexes/serum biochemical indexes/ser⁃um immune indexes分类
农业科技引用本文复制引用
贺绍君,吴明明,宋凯,丁金雪..短期冰水刺激对犬血常规、血清生化和免疫指标的影响[J].动物营养学报,2019,31(11):5143-5151,9.基金项目
国家自然科学基金青年基金项目(31702306) （31702306）
安徽省科技厅自然科学研究项目(1508085QC61) （1508085QC61）
安徽省教育厅自然科学重大项目( KJ2018ZD052) （ KJ2018ZD052）
