动物营养学报2019，Vol.31Issue(11)：5179-5184,6.DOI:10.3969/j.issn.1006⁃267x.2019.11.033
叶酸对铅暴露大鼠肝脏氧化损伤的影响
Effects of Folic Acid on Liver Oxidative Damage in Lead?Exposed Rats
摘要
Abstract
The aim of this study was to investigate the effects of folic acid on lead?induced oxidative stress and nuclear factor E2?related factors ( Nrf2) and heme oxygenase?1 ( HO?1) protein expression in rat liver. Forty 2?month?old male rats were randomly divided into 4 groups ( n＝10) and treated as follows: control group, free drinking water+1 mL deionized water was intragastrically administered; lead group, free drink 0.2% lead acetate solution+1 mL deionized water was intragastrically administered; intervention group, free drink 0.2%lead acetate solution+1 mL folic acid suspension was intragastrically administered ( prepared according to the dose of 0.4 mg／kg BW folic acid) ; folic acid group, free drinking water+1 mL folic acid suspension was in?tragastrically administered ( prepared according to the dose of 0.4 mg／kg BW folic acid). Rats in each group were given intragastric administration once a day. After 14 days, the activities of superoxide dismutase ( SOD) , glutathione peroxidase ( GSH?Px) and the contents of malondialdehyde ( MDA) and reduced gluta?thione ( GSH) in rat liver were determined by corresponding kits, and the expression levels of Nrf2 and HO?1 proteins in liver were determined by immunohistochemistry. The results showed that compared with the control group, the liver GSH content and GSH?Px activity in the lead?exposed group were significantly decreased ( P＜0.05) , while the serum lead content and the MDA content and the expression levels of HO?1 and Nrf2 proteins in liver were significantly increased ( P＜0.05). Compared with the lead?exposed group, the MDA content and the expression levels of Nrf2 and HO?1 proteins in liver of the intervention group were significantly decreased ( P＜0.05). Compared with the control group, the serum lead content in the intervention group was significant?ly increased ( P＜0.05) , and there were no significant changes in SOD activity, GSH and MDA contents and Nrf2 and HO?1 protein expression levels in liver ( P＞0.05).In addition, the activity of GSH?Px in liver of folic acid group was significantly higher than that of the control group ( P＜0.05). It is concluded that folic acid has a certain degree of protective effect on liver oxidative damage in lead?exposed rats.[ Chinese Journal of Animal Nutrition, 2019, 31(11) :5178?5183]关键词
叶酸/铅/肝脏/氧化活性/核因子E2相关因子/血红素氧合酶－1Key words
folic acid/lead/liver/oxidative activity/Nrf2/HO⁃1分类
农业科技引用本文复制引用
李宁,赵紫薇,张凡,曹帅,沈玥,张迪,宋莲军,黄现青,乔明武,张平安,王凡..叶酸对铅暴露大鼠肝脏氧化损伤的影响[J].动物营养学报,2019,31(11):5179-5184,6.基金项目
国家自然科学基金项目(31201878,U1604183) （31201878,U1604183）
河南省重点研发与推广专项(科技攻关) (182102310252) （科技攻关）
河南农业大学大学生实践创新训练计划项目(201910466024) （201910466024）
