动物营养学报2019，Vol.31Issue(11)：5203-5214,12.DOI:10.3969/j.issn.1006⁃267x.2019.11.036
基于转录组测序筛选维生素 D调节肉鸡十二指肠钙和磷吸收的差异表达基因
Differentially Expressed Genes in Vitamin D Regulating Calcium and Phosphorus Absorption in Duodenum of Broiler Chickens Using RNA?Sequencing
摘要
Abstract
This study was conducted to investigate the candidate genes and signaling pathways in vitamin D regulating calcium and phosphorus absorption in small intestine of broiler chickens using RNA?sequencing tech?nology. Ross 308 broiler chickens at 1 to 16 days of age were used as experimental animals. Three levels [ 0 ( control group) , 5 and 10 μg／kg] of 1,25?dihydroxycholecalciferol [ 1,25?( OH) 2?D3 ] were added to the basal diet without vitamin D. Broiler chickens were killed and duodenal mucosa was collected at 16 days of age. Gene ontology ( GO) function annotation and kyoto encyclopedia of genes and genomes ( KEGG) signal pathway enrichment of differentially expressed genes were carried out using RNA?sequencing technology. Re?sults showed as follows: 1) compared with the control group, the addition of 5 and 10 μg／kg of 1,25?( OH) 2?D3 increased significantly growth performance ( P＜0.05) and improved tibia and femur mineralization. The weight, length and ash content of tibia and femur of broilers in 10 μg／kg of 1,25?( OH) 2?D3 group were sig?nificantly lower than those in 5 μg／kg 1,25?( OH) 2?D3 group ( P＜0.05). 2) A total of 1 029, 939, and 642 differentially expressed genes were screened when comparing the three levels of 1, 25?( OH ) 2?D3 ( 0 vs. 5 μg／kg, 0 vs. 10 μg／kg, and 5 vs. 10 μg／kg) , respectively. 3) GO analysis identified candidate genes reg?ulating calcium and phosphorus absorption and metabolism, including nuclear vitamin D receptor ( VDR) , pro?tein disulfide?isomerase A3 ( PDIA3) , type Ⅱb sodium phosphate transporter protein NaPi?Ⅱb ( SLC34A2) , inorganic phosphorus transporter carrier protein 2 ( SLC20A2 ) , calbindin D28k ( CALB1 ) , ATPase plasma membrane Ca2+ transporting 1 ( ATP2B1) , ATPase plasma membrane Ca2+ transporting 2 ( ATP2B2) , solute carrier family 8 member A1 NCX1 ( SLC8A1) , fibroblast growth factor 1 ( FGF1) and fibroblast growth fac?tor 19 ( FGF19). 4) KEGG analysis indicated pathways associated with the intestinal calcium and phosphorus absorption and metabolism of broiler chickens, including mitogen?activated protein kinase ( MAPK) signaling pathway, cyclic adenosine monophosphate ( cAMP) signaling pathway or cyclic adenosine monophosphate?protein kinase A ( cAMP?PKA ) signaling pathway, and cyclic guanosine monophosphate?protein kinase G ( cGMP?PKG) signaling pathway. 5) The results of qRT?PCR were in accordance with those of RNA?seq. In summary, the differentially expressed genes and signaling pathways in vitamin D regulating calcium and phos?phorus absorption in small intestine of broiler chickens are screened. These data will be helpful to improve cal?cium and phosphorus absorption and retention in poultry diets.[ Chinese Journal of Animal Nutrition, 2019, 31(11) :5202?5213]关键词
肉鸡/十二指肠/钙磷代谢/差异表达基因/信号通路Key words
broiler chicken/duodenum/calcium and phosphorus metabolism/differentially expressed gene/signaling pathway分类
农业科技引用本文复制引用
杨雪,韩进诚,汪晓娜,张宁,瞿红侠,施传信,张进良,闫永峰,郑永祥,王志祥..基于转录组测序筛选维生素 D调节肉鸡十二指肠钙和磷吸收的差异表达基因[J].动物营养学报,2019,31(11):5203-5214,12.基金项目
国家自然科学基金项目( U1704107) （ U1704107）
