动物营养学报2019，Vol.31Issue(11)：5215-5221,7.DOI:10.3969/j.issn.1006⁃267x.2019.11.037
5－羟色胺前体物对围产期母羊肝脏糖异生关键基因 mRNA和蛋白质表达量的影响
Effects of Serotonin Precursor on mRNA and Protein Expressions of Liver Gluconeogenesis Key Genes of Perinatal Ewes
摘要
Abstract
This study was conducted to investigate the effects of serotonin ( 5?HT) precursor on mRNA and protein expressions of liver gluconeogenesis key genes of perinatal ewes. Thirty good body condition Bamei ewes with a body weight of (64.52±4.11) kg in the same perinatal period were randomly divided into 3 groups according to their body weight, which were control group, 5?hydroxy?tryptophan ( 5?HTP) group ( perfused 5?HTP) and tryptophan ( Trp) group ( perfused Trp) , respectively, each group contained 10 ewes. Ewes jug?ular vein perfusion was conducted from prenatal day 7 to postpartum day 0, the perfusion dose of 5?HTP and Trp was 0.178 mg／kg BW, and the concentration was 0.1 mg／mL; the control group was perfused with the same dose of saline. The experiment lasted form prenatal day 7 to postpartum day 30. The results showed as follows: 1) the mRNA expressions of phosphoenolpyruvate carboxykinase ( PEPCK) , glucose?6?phosphatase ( G6P) and pyruvate carboxylase ( PC) in liver of 5?HTP group and Trp group were significantly higher than those of the control group ( P＜0.05). 2) The protein expressions of PEPCK, G6P and PC in liver of 5?HTP group and Trp group were significantly higher than those of the control group ( P＜0.05) , the liver PEPCK pro?tein expression of Trp group was significantly lower than that of 5?HTP group ( P＜0.05) , and the liver G6P protein expression of Trp group was significantly higher than that of 5?HTP group ( P＜0.05). In conclusion, 5?HT precursor plays a role in promoting the liver gluconeogenesis not only at the transcriptional level, but also at the translation level.[ Chinese Journal of Animal Nutrition, 2019, 31(11) :5214?5220]关键词
5－羟色胺前体物/肝脏糖异生/围产期/母羊Key words
serotonin precursor/liver gluconeogenesis/perinatal period/ewes分类
农业科技引用本文复制引用
桑丹,娜美日嘎,孙海洲,金鹿,张崇志,张春华,茹婷,贾春雷,敖长金..5－羟色胺前体物对围产期母羊肝脏糖异生关键基因 mRNA和蛋白质表达量的影响[J].动物营养学报,2019,31(11):5215-5221,7.基金项目
内蒙古农牧业科技创新基金项目(2018CXJJM04) （2018CXJJM04）
国家绒毛用羊产业技术体系( CARS?39?11) （ CARS?39?11）
内蒙古自治区科技成果转化项目( CGZH2018166) （ CGZH2018166）
公益性行业(农业)科研专项(201303062) （农业）
