动物营养学报2019，Vol.31Issue(11)：5368-5378,11.DOI:10.3969/j.issn.1006⁃267x.2019.11.054
山羊急性瘤胃酸中毒前后瘤胃菌群结构与组成变化分析
Analysis on Structure and Composition of Rumen Bacterial Flora before and after Acute Rumen Acidosis in Goats
摘要
Abstract
Rumen microecological balance is an important guarantee for goat health. This study aimed to ana?lyze the changes of rumen bacterial flora before and after acute rumen acidosis in goats by using 16S rRNA se?quencing technology. Four healthy Boer goats were selected, and the self?designed before?after control experi?ment design was adopted. Four portions of tumor?producing fluid collected before modeling were set as healthy group, and four portions of tumor?producing fluid collected after modeling were set as rumen acidosis group. The goat acute rumen acidosis model was established by infusing corn flour, and the amount of corn flour was determined by the ratio of 40 g／kg BW. The method of rumen intubation was used for the collection of gastric juice and corn flour irrigation. The results showed that the diversity and similarity of rumen bacteria flora de?creased after acute rumen acidosis. Streptococcus and Lactobacillales under Firmicutes became the dominant bacteria flora, Succinivibrionaceae and Mitochondria under Proteobacteria grew moderately, Prevotella under Bacteroidetes showed no significant difference in relative abundance after acute rumen acidosis ( P＞0.05 ). LEfSe analysis showed that Fibrobacteraceae under Fibrobacteres became one of the influencing factors of the difference of rumen bacterial flora composition before and after acute rumen acidosis in goats. PICRUSt analy?sis found that the rumen bacteria flora may maintain normal survival and reproduction by increasing the utiliza?tion of carbohydrates and amino acids when the functions of protein synthesis, processing and degradation were inhibited. It can be seen that the composition, structure and function of rumen bacteria flora have obvious chan?ges compared with the healthy state after the induced acute rumen acidosis in goats.[ Chinese Journal of Ani?mal Nutrition, 2019, 31(11) :5367?5377]关键词
山羊/急性瘤胃酸中毒/瘤胃菌群/16S rRNAKey words
goat/acute rumen acidosis/rumen bacterial flora/16S rRNA分类
农业科技引用本文复制引用
李小玉,冯士彬,王希春,吴金节,孙裴,李玉,苏思毅,钟富超,黄莹莹,刘蕾红,李稳稳,林家辉,李锦春,刘亚..山羊急性瘤胃酸中毒前后瘤胃菌群结构与组成变化分析[J].动物营养学报,2019,31(11):5368-5378,11.基金项目
国家重点研发计划( 2018YFD0502006 ) （ 2018YFD0502006 ）
国家自然科学基金项目( 31873029 ) （ 31873029 ）
安徽省重点研究与开发计划项目(1804g07020187) （1804g07020187）
安徽省现代农业牛羊产业技术体系( AHCYJSTX?07) （ AHCYJSTX?07）
安徽农业大学2018年校级质量工程项目(2018aujyxm023) （2018aujyxm023）
