南方医科大学学报2019，Vol.39Issue(12)：1387-1392,6.DOI:10.12122/j.issn.1673-4254.2019.12.01
奇任醇通过抑制炎症细胞因子和诱导淋巴细胞凋亡减轻小鼠溃疡性结肠炎
Kirenol relieves dextran sulfate sodium-induced ulcerative colitis in mice by inhibiting inflammatory cytokines and inducing CD4+T lymphocyte apoptosis
摘要
Abstract
Objective To investigate whether kirenol, the major pharmacologically active compound of the Chinese medicinal herb Herba Siegesbeckiae, can protect mice from dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). Methods C57BL/6 mice with or without kirenol pretreatment were treated with DSS in drinking water for 7 days to induce UC. The symptoms of UC including weight loss, diarrhea and bloody stool were observed daily and graded using the disease activity index (DAI). Colon injury of the mice was assessed by measuring the length of the colon and HE staining of the colon tissue. The levels of inflammatory cytokines produced by the mesenteric lymph nodes (MLNs) lymphocytes were measured using enzyme-linked immunosorbent assay; the apoptosis of the lymphocytes and CD4 + T cells was analyzed using flow cytometry. Results The mice receiving pretreatment with kirenol showed obviously ameliorated symptoms of UC and milder pathological changes in the colon as compared with the control mice. Kirenol treatment significantly down-regulated the secretion of IFN-γ, IL-17A, IL-6 and TNF-α by the MLNs lymphocytes and increased the apoptosis of lymphocytes, especially CD4 + T cells in the DSS-treated mice. Conclusion Kirenol can protect against T cell-mediated colon injury in DSS-treated mice possibly by suppressing the secretion of inflammatory mediators and inducing apoptosis of the inflammatory lymphocytes.关键词
奇任醇/溃疡性结肠炎/淋巴细胞/凋亡Key words
kirenol/ulcerative colitis/lymphocytes/apoptosis引用本文复制引用
刘秀红,杜亚军,刘国星,但国梅,童鑫,肖娟..奇任醇通过抑制炎症细胞因子和诱导淋巴细胞凋亡减轻小鼠溃疡性结肠炎[J].南方医科大学学报,2019,39(12):1387-1392,6.基金项目
国家自然科学基金(81601373) （81601373）
国家留学基金,2019年襄阳市青年科技晨光计划,襄阳市青年科技英才开发计划(No.[2018]46)Supported by National Natural Science Foundation of China(81601373) （No.[2018]46）
National Fund for Studying Abroad, Xiangyang Youth Science and Technology Morning Plan 2019, Xiangyang Youth Science and Technology Talent Development Plan (No.[2018]46). （No.[2018]46）
