医学分子生物学杂志2019，Vol.16Issue(6)：487-493,7.DOI:10.3870/j.issn.1672-8009.2019.06.001
miR-20b通过靶向VEGFA对鼻咽癌细胞增殖凋亡和侵袭的调控作用
Regulatory Effect of miR-20b on the Proliferation, Apoptosis and In-vasion of Nasopharyngeal Carcinoma Cells by Targeting VEGFA
摘要
Abstract
Objective To explore the regulatory mechanism of miR-20b on the proliferation, apoptosis and invasion of nasopharyngeal carcinoma ( NPC) cells by targeting VEGFA.Methods RT-PCR was applied to detect the expression levels of miR-20b and VEGFA in NPC tissue samples and cell lines.NPC CNE cells were divided into blank control group, negative control group and miR-20b transfection group.Cells in the negative control group and miR-20b transfection group were transfected with blank plasmid and miR-20b, respectively.The expression level of VEGFA gene in each group was detected by RT-PCR.The luciferase reporter gene test was applied to analyze the tar-geting relationship between miR-20b and VEGFA.NPC CNE cells were divided into negative control group, miR-20b transfection group, VEGFA group and miR-20b +pc-VEGFA group.The expres-sion of VEGFA protein in each group was detected by Western blotting.Cell proliferation was detec-ted by CCK-8.Apoptosis was detected by flow cytometry.The expression of proteins related to cell proliferation, apoptosis and invasion was detected by Western blotting.Cell invasion was detected by Transwell method. Results The expression level of miR-20b in NPC tissue samples was signifi-cantly lower than that in normal tissues, while expression of VEGFA was significantly higher than that in normal tissues ( P <0.05 ).The luciferase reporter assay showed that there were binding sites of VEGFA on miR-20b.Compared with those in the negative control group, the miR-20b ex-pression, apoptosis rate, expression of Bax and E-cadherin were significantly increased, while the VEGFA expression, cell proliferation rate, expression of Ki67, PCNA and Bcl-2, cell invasive a-bility, expression of vimentin and fibronectin were significantly decreased in miR-20b transfection group (P <0.05 ).Compared with those in VEGFA group, the miR-20b expression, apoptosis rate, expression of Bax and E-cadherin were significantly increased, while the VEGFA expression, cell proliferation rate, expression of Ki67, PCNA and Bcl-2, cell invasive ability, expression of vimentin and fibronectin were significantly decreased in miR-20b+pc-VEGFA group ( P<0.05) . Conclusion miR-20b can promote the apoptosis of NPC cells and inhibit their proliferation, inva-sion and migration by targeting VEGFA.关键词
鼻咽癌/miR-20b/细胞增殖/细胞凋亡/细胞侵袭Key words
nasopharyngeal carcinoma/MiR-20b/cell proliferation/apoptosis/cell invasion分类
医药卫生引用本文复制引用
田妍妍,董艳,张昌明,冯瑞..miR-20b通过靶向VEGFA对鼻咽癌细胞增殖凋亡和侵袭的调控作用[J].医学分子生物学杂志,2019,16(6):487-493,7.基金项目
陕西省社会发展重点研发计划 (No.2017 ZDXM-SF-037) This work was supported by a grant from the Key Research and Development Plan of Social Development in Shaanxi Province (No.2017 zdxm-sf-037) （No.2017 ZDXM-SF-037）
