医学分子生物学杂志2019，Vol.16Issue(6)：531-536,6.DOI:10.3870/j.issn.1672-8009.2019.06.008
DCK基因敲减对宫颈癌HeLa细胞增殖、侵袭及放化疗敏感性的影响
Impact of DCK Knockdown on Proliferation, Invasion and Response to Chemoradiotherapy in Cervical Cancer HeLa Cells
摘要
Abstract
Objective To investigate the effect of DCK knockdown on cell proliferation, inva-sion and response to chemoradiotheray in cervical cancer HeLa cells.Methods DCK expression was reduced by siRNA in HeLa cells.CCK-8 assay, colony formation assay and invasion assay were used to evaluate the proliferation rate, colony formation and invasion ability of HeLa cells with or without treatment of DCK siRNA.Different dosage of cisplatin and X-rays were used for the treatment of control and DCK knowckdown cells, and CCK-8 assay was then used to evaluate their prolifera-tion ability.By using autophagy inhibitor 3-methyladenine and inducer rapamycin, and different dos-age of X-rays, CCK-8 assay and westernblot assay were used to measure proliferation rate and LC-Ⅱ expression in control and DCK knockdown cells.Results The proliferation rate, colony forma- tion and invasion ability was significant inhibited by DCK knockdown ( P<0.01) ; For the cispla-tin treatment, the proliferation of control cells significant decreased in a dose dependent manner, however there is no different of growth rate between control and DCK knockdown cells ( P>0.05) . For the X-ray treatment, the proliferation rate of control cells significant decreased in a dose de-pendent manner, and the growth rate is further inhibited in DCK knockdown cells compared to con-trol cells (P<0.01) .For the treatment of X-rays and DCK knockdown, 3-methyladenine caused further growth inhibitory ( P<0.01) , while rapamycin is able to reverse growth inhibitory induced by DCK knockdown ( P<0.01) .For the treatment of 2 Gy and 6 Gy of X-rays, the expression level of LC-Ⅱ is significantly induced in control cells, while no obvious change was observed in DCK knockdown cells.Conclusion DCK plays an important role in proliferation and invasion in cervical cancer cells.Knockdown of DCK caused increased response to radiotherapy in cervical canc-er.关键词
宫颈癌/DCK/顺铂/放射/细胞自噬Key words
cervical cancer/DCK/cisplatin/radiotherapy/autophagy分类
医药卫生引用本文复制引用
王健,吴丛山,高华荣,成昌宏,尚庆毅..DCK基因敲减对宫颈癌HeLa细胞增殖、侵袭及放化疗敏感性的影响[J].医学分子生物学杂志,2019,16(6):531-536,6.基金项目
江苏省连云港市卫生和计划生育委员会 (No.201836) This work was supported by a grant from the Health and Family Planning Commission of Lianyungang City, Jiangsu Province (No.201836) （No.201836）
