王锋 1朱瑾 1张素雅 1郭思思 1周叶 1张怀壁 1朱静嫣 1胡春梅1
作者信息
- 1. 上海市宝山区中西医结合医院神经内科 上海市, 201901
- 折叠
摘要
Abstract
Objective To investigate the neuroprotective effect of green tea polyphenols (GTP) on Parkinson’s disease ( PD) rats and its mechanism.Methods The rats were divided into Control group, Model group, GTP low, medium, and high dose groups ( GTP-L, GTP-M, GTP-H), PI3K activator group (740 Y-P), with 12 rats in each group.The neurobehavioral chan-ges of rats were evaluated by apomorphine ( APO) rotation induction test and suspension test.The positive expression of thyrosine hydroxylase ( TH) in substantia nigra of rats was detected by immu-nohistochemistry.The activities of malondialdehyde ( MDA ) , superoxide dismutase ( SOD ) and glutathione peroxidase ( GSH-Px ) in substantia nigra were detected.The positive expression of CD11b was detected by immunofluorescence.Tunel staining was used to detect neuronal apopto-sis.The expression levels of phosphatidylinositol kinase ( PI3K ) , p-phosphatidylinositol kinase ( PI3K), protein kinase B ( Akt) , P-protein kinase B ( Akt) , Bcl-2, Bax, and Caspase-3 were detected by Western blotting ( WB).Results Compared with Sham group, APO induced cycles number increased, hanging test score increased, TH positive expression rate in substantia nigra de- creased, SOD, GSH-PX levels decreased and MDA levels increased in substantia nigra, the posi-tive expression rate of CD11b increased, the expression of p-PI3K and p-Akt decreased, and the apoptotic rate of nerve cells increased, the expression of Bax and caspase-3 protein increased, the expression of Bcl-2 protein decreased in Model group, and the differences were significant ( all P<0.05).Compared with model group, APO induced cycles number decreased, hanging test score decreased, the levels of SOD and GSH-PX increased and MDA decreased in substantia nigra, the positive expression rate of CD11b decreased, the expressions of p-PI3K and p-Akt increased, and the apoptotic rate of nerve cells decreased, the expression levels of Bax and caspase-3 decreased, while the expression of Bcl-2 increased in GTP group and 740 Y-P group, and the differences were significant ( all P<0.05).Conclusion Green tea polyphenols may play a neuroprotective role by activating PI3K/Akt signaling pathway, reducing oxidative stress response, and alleviating neuronal damage.关键词
帕金森/绿茶多酚/磷脂酰肌醇酶/蛋白激酶B/神经保护Key words
Parkinson/green tea polyphenols/phosphatidylinositol kinase/protein kinase B/neuroprotection分类
医药卫生
