医学分子生物学杂志2020，Vol.17Issue(1)：33-39,7.DOI:10.3870/j.issn.1672-8009.2019.01.006
柴胡皂苷D通过下调miR-517a调节胶质瘤细胞体外和体内移植瘤生长
Saikosaponin D Regulates Growth of Glioma Cells in vitro and Trans-planted Tumor in vivo by Down-regulating miR-517a
摘要
Abstract
Objective To investigate the effect of Saikosaponin D on the growth of glioma cells in vitro and in vivo by regulating miR-517a.Methods Survival rates of U-87 cells and normal astro-cytes were determined by MTT kit.miR-517a expression was detected by q-PCR.Cell apoptosis was assessed by flow cytometry.Transwell assay was applied to detect cell migration and invasion.CCK-8 kit was used to detect cell proliferation.Cleaved caspase-3, Ki67, MMP-2 and Cyclin D1 protein levels were detected by Western blotting.The model of transplanted tumor in nude mice was estab-lished and the weight of tumor in each group was detected.The expression levels of Ki67 and VEGF were detected by immunohistochemistry.Results Saikosaponin D had no effect on the survival rate of normal astrocytes, decreased the survival rate of U-87 cells, down-regulated the expression of miR-517a, promoted apoptosis, up-regulated cleaved caspase-3 protein expression, inhibited cell migration, invasion and proliferation, and down-regulated MMP-2, Cyclin D1 and Ki67 protein expression.The growth of transplanted tumor was inhibited, and the positive expression rates of Ki67 and VEGF were de-creased by Saikosaponin D.Conclusion This study shows that Saikosaponin D can down-regulate miR-517a to regulate the growth of glioma cells in vitro and transplanted tumor in vivo.关键词
柴胡皂苷D/ 胶质瘤/ miR-517a/ 血管内皮生长因子Key words
SaikosaponinD/glioma/miR-517a/VEGF分类
医药卫生引用本文复制引用
徐敬轩,杨岩,张勖,郭正,栾新平..柴胡皂苷D通过下调miR-517a调节胶质瘤细胞体外和体内移植瘤生长[J].医学分子生物学杂志,2020,17(1):33-39,7.基金项目
资助项目:新疆维吾尔自治区自然科学基金(No.2019001C234) This work was supported by a grant from the Nature Science Foundation of Xinjiang Uygur Autonomous Region(No.2019001C234) （No.2019001C234）
