烟草科技2019，Vol.52Issue(12)：17-22,6.
烟草主要病害拮抗菌的筛选鉴定及除草活性分析
Screening and identification of antagonistic Trichoderma sp. herbicidal activity against main tobacco diseases
摘要
Abstract
In order to screen herbicide strains with good control over common tobacco diseases and weeds, marine mangrove-derived Trichoderma sp. was isolated and purified. The isolated Trichoderma sp. strains were identified according to morphological characteristics combined with molecular biology, and their antimicrobial as well as herbicidal activities were analyzed. The results showed that there were 17 Trichoderma asperellum strains, including 3 T. harzianum strains, 2 T. hamatum strains, 2 T. reesei strains and 1 T. koningiopsis strain. Among the above 25 strains, 1 T. reesei strain (HT6), 1 T. harzianum strain (HT8), 1 T. koningiopsis strain (HT11), and 10 T. asperellum strains (HG2, HG9, HG11, HG14, HG16, 9HG, HJ3, 5HJ, HJ7, HT2) presented significant inhibitory effects on Phytophthora parasitica var. nicotianae with the inhibition rate of more than 60% ; 4 T. asperellum strains (HG9, HG11, HG16, HT3) and 1 T. koningiopsis strain (HT11) had inhibition effect against Alternaria alternata (Fries) Keissler with the inhibition rate of more than 40% ; 2 T. reesei strains (HT6 and HT7) possessed inhibitory activity against Ralstonia solanacearum; and 1 T. harzianum strain (HT10) had herbicidal activity against amaranth (Amaranthus retroflexus L.).关键词
烟草/海洋木霉/黑胫病/赤星病/青枯病/除草活性Key words
Tobacco/Marine-derived Trichoderma/Phytophthora parasitica var. nicotianae/Alternaria alternate (Fries) Keissler/Ralstonia solanacearum/Herbicidal activity分类
农业科技引用本文复制引用
黄瑞环,芶剑渝,韩小斌,刘京,彭玉龙,王丹,张明岳,张成省,赵栋霖..烟草主要病害拮抗菌的筛选鉴定及除草活性分析[J].烟草科技,2019,52(12):17-22,6.基金项目
国家自然科学基金项目"多级联合筛选及表观遗传修饰策略下海洋真菌新颖抗青枯菌产物研究"(41806194) （41806194）
中国烟草总公司贵州省公司科技项目"海洋真菌来源天然生物除草剂的发现及应用"(201710) （201710）
贵州省烟草公司遵义市公司项目"基于OSMAC策略的海洋真菌来源生物除草剂的研究"(2018-03) （2018-03）
中央级公益性科研院所基本科研业务费专项"沿海滩涂真菌资源多样性及其活性级代谢产物研究"(1610232017013) （1610232017013）
中国农业科学院烟草研究所青年科学基金"一株海洋滩涂来源真菌Alternaria sp.W9抗菌活性产物研究"(2017B08). （2017B08）
