烟草科技2019，Vol.52Issue(12)：31-38,8.
UPLC-MS/MS法测定胶基型嚼烟中13种甜味剂
Determination of thirteen sweeteners in gum-based chewing tobacco with UPLC-MS/MS method
摘要
Abstract
To rapidly determine seven synthetic sweeteners (Acesulfame potassium, Sodium cyclamate, Saccharin sodium, Aspartame, Neotame, Sucralose and Stevioside) and six natural sweeteners (Erythritol, Xylitol, Mannitol, Sorbitol, Maltitol and Isomaltitol) in gum-based chewing tobacco, an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed. Samples of gum-based chewing tobacco were extracted by shaking with n-hexane and water. The seven synthetic sweeteners were separated by reversed phase column using gradient methanol/water as mobile phase. The six natural sweeteners were separated by special sugar column using gradient acetonitrile/water solution as mobile phase. All the sweeteners were analyzed by UPLC-MS/MS under the negative ion monitoring mode. The results showed that: 1) The linear correlation of the thirteen sweeteners were good (R2＞0.994), the limits of detection and quantitation were 6.8×10-6-0.30 and 2.3×10-5-1.0 μg/kg, respectively; the linear ranges of all the sweeteners ranged from 0.001 to 1.0 μg/mL. 2) The recoveries of the sweeteners were from 80.2% to 97.6%. 3) The precisions of intra- and inter-day were all less than 10% (n=6). This method is suitable for the rapid qualitative and quantitative analysis of sweeteners in gum-based chewing tobacco.关键词
胶基型嚼烟/超高效液相色谱-串联质谱/合成甜味剂/天然甜味剂Key words
Gum-based chewing tobacco/Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)/Synthetic sweetener/Natural sweetener分类
轻工纺织引用本文复制引用
付强,李国政,林奕云,杨继,蔡群娣,张东豫,邱建华..UPLC-MS/MS法测定胶基型嚼烟中13种甜味剂[J].烟草科技,2019,52(12):31-38,8.基金项目
广东省自然科学基金"新型聚合物整体柱的制备与在线固相萃取-液质联用农兽药分析方法学研究"(2015A030310466). （2015A030310466）
