烟草科技2019，Vol.52Issue(12)：39-49,11.
GC-MS/MS法测定烟草中的57种酯类香味成分
Determination of 57 ester aroma components in tobacco by gas chromatography-tandem mass spectrometry
赵嘉幸 1陈黎 1任宗灿 1王晓瑜 1崔华鹏 1谢复炜 1刘惠民1
作者信息
- 1. 中国烟草总公司郑州烟草研究院,郑州高新技术产业开发区枫杨街2号 450001
- 折叠
摘要
Abstract
To detect the ester aroma components in tobacco more accurately and rapidly, a gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed on the basis of acidic solution soaking, acetonitrile extraction and anhydrous magnesium sulfate dehydration,and 57 key ester aroma components in tobacco were analyzed simultaneously. The mass spectrometry parameters were optimized, and the effects of extraction type, extraction solvent, pH value of soaking solution, soaking time, vortex time and purification method on extraction efficiency were investigated. The samples of 15 types of cigarettes of different styles were analyzed by the developed method. The results showed that: 1) The calibration curve of all the esters presented good linearity when quantitatively analyzing under multiple reaction monitoring mode (dMRM). The average recoveries ranged from 62.5% to 128.7% with the relative standard deviations between 0.5% and 25.4% at three spiked levels. The limits of quantitation (LOQs) were in the range of 3-74 μg/kg. 2) This method was accurate, sensitive, convenient and met the needs of analysis of ester aroma components in tobacco. 3) Thirty-one ester aroma components were detected in cigarette samples, in which 8 components with significant differences were screened out by t test. The results of Partial Least Square Discriminant Analysis (PLS-DA) indicated that the selected indexes could distinguish cigarette samples of different styles.关键词
烟草/酯类化合物/香味成分/气相色谱-串联质谱/偏最小二乘判别分析Key words
Tobacco/Ester/Aroma component/GC-MS/MS/PLS-DA分类
轻工纺织引用本文复制引用
赵嘉幸,陈黎,任宗灿,王晓瑜,崔华鹏,谢复炜,刘惠民..GC-MS/MS法测定烟草中的57种酯类香味成分[J].烟草科技,2019,52(12):39-49,11.
