中国烟草科学2019，Vol.40Issue(6)：26-32,7.DOI:10.13496/j.issn.1007-5119.2019.06.004
烟秆高效复合降解菌群的筛选、鉴定及组合应用
Screening, Identification and Combination of High Efficiency Degrading Bacteria for Tobacco Stem
摘要
Abstract
In order to improve the degradation efficiency of lignin and cellulose in tobacco straw and shorten the decomposing time of tobacco straw, the strains with the ability to degrade lignin and cellulose were screened out by plate screening and enzyme activity determination experiments. The effects of different strain combinations on tobacco straw degradation were discussed. The results showed that the obtained Lysinibacillus fusiformis (N019a), Trametes hirsuta (MA), and Rhizopus oryzae (imp) were effective in degrading lignin, hemicellulose, and cellulose in tobacco straw. The composite flora I composed of N019a, MA and imp was used for solid fermentation of tobacco stalk powder for 25 d. The degradation rates of hemicellulose, cellulose and lignin were 67.23±0.73%, 61.17±0.45% and 60.12±0.48%, respectively. B. tequilensis (B4), B. subtilis (B26) and A. niger (M90) composed a complex flora II with degradation rates of hemicellulose, cellulose and lignin being 72.15±0.35%, 65.68±0.55%, and 38.15±0.76%. The degradation rates of hemicellulose, cellulose and lignin by compound flora III composed of 6 strains was were 83.32±0.45%, 75.21±0.71% and 66.13±0.53%. Different degrading bacteria degrade different components in wood fiber with certain specificity, and the combination of multifunctional strains can significantly improve the biomass degradation effect of tobacco straw. The research results provide a basis for the development of a highly effective compound bacterial agent for tobacco straw degradation.关键词
烟草秸秆/复合菌群/木质素/纤维素Key words
tobacco straw/ composite flora/ lignin/ cellulose分类
农业科技引用本文复制引用
李春黎,李文豪,余君,孙敬国,王昌军,陈守文,杨勇..烟秆高效复合降解菌群的筛选、鉴定及组合应用[J].中国烟草科学,2019,40(6):26-32,7.基金项目
湖北省烟草公司重点科技项目"烟草秸秆生物有肥快速发酵技术研究与应用"(027Y2015-004) （027Y2015-004）
