中国烟草科学2019，Vol.40Issue(6)：49-54,6.DOI:10.13496/j.issn.1007-5119.2019.06.007
云南省大理州烟草疫霉交配型及甲霜灵敏感性研究
Mating Type and Metalaxyl Sensitivity of Phytophthora nicotianae on Tobacco Black Shank Disease in Dali, Yunnan
摘要
Abstract
In order to investigate population structure and metalaxyl resistance level of Phytophthora nicotianae on tobacco black shank disease, the methods of confrontation culture and fungicide-amended plate were used to determine mating type and sensitivity to metalaxyl of P. nicotianae isolates collected from 2013 to 2014 in Dali, Yunnan Province. The results showed that all 150 isolates of P. nicotianae from 12 counties were A2 mating type and A1 or A0 mating type was not identified. The EC50 values of a total of 263 isolates to metalaxyl distributed between 1.0829 and 33.6227 mg/L, with an average EC50 value of 8.7599±0.0358 mg/L. The ratio of sensitive, moderate resistant and resistant population was 14.83%, 52.85% and 32.32%, respectively, which means that sensitivity to metalaxyl of P. nicotianae isolates collected from Dali had significant differences. Our results suggest that the population structure of P. nicotianae was mainly maintained through asexual propagation in its life cycle in Dali. The pathogen had a high resistance level to metalaxyl and both moderate and resistant isolates were widely distributed. It is urgent to screen and make use of new high-efficiency and safe alternatives for black shank disease management.关键词
烟草黑胫病/烟草疫霉/交配型/甲霜灵敏感性Key words
tobacco black shank disease/ Phytophthora nicotianae/ mating type/ metalaxyl sensitivity分类
农业科技引用本文复制引用
曹继芬,霍超,户艳霞,王新中,徐发华,赵志坚..云南省大理州烟草疫霉交配型及甲霜灵敏感性研究[J].中国烟草科学,2019,40(6):49-54,6.基金项目
中国烟草总公司云南省公司科技项目"大理州红花大金元两黑病持续控制技术研究及应用"(2013YN27) （2013YN27）
