中国卒中杂志2019，Vol.14Issue(12)：1225-1231,7.DOI:10.3969/j.issn.1673-5765.2019.12.006
丁基苯酞对脑出血大鼠血管内皮生长因子、血管生成素-2蛋白表达及血管新生的影响
The Effect of Dl-3-n-butylphthalide on Expressions of VEGF, Ang-2 Proteins and Angiogenesis in Intracerebral Hemorrhage Rats
摘要
Abstract
investigate the possible neuroprotective effect and mechanism of dl-3-n-butylphthalide in intracerebral hemorrhage rats by observing the dynamic expression of vaseular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) protein and the change of neovascularization in brain tissue of rats with intracerebral hemorrhage at different time points after giving different doses of dl-3-n-butylphthalide. Methods Male healthy SD rats were used to prepare cerebral hemorrhage models by injecting autologous blood, and were randomly divided into no-treatment intracerebral hemorrhage model group, the low-dose dl-3-n-butylphthalide group and medium-dose dl-3-n-butylphthalide group, and the sham-operated rats served as control group. Rats in the low and medium dose groups were given dl-3-n-butylphthalide in the dose of 10 mg/kg and 25 mg/kg (twice a day for both groups) respectively, while rats in the sham operation group and no-treatment intracerebral hemorrhage model group were given the same dose of soybean oil at the same time. The following indexes in all groups were assessed at 1, 3, 7 and 15 day after operation: neurological deficit, the expression of CD34, neovascularization and vascular field area, the expressions of VEGF and Ang-2 protein. The volume of hematoma in intracerebral hemorrhage rats were also measured. Results Compared with that in no-treatment model group, the expression of VEGF was up-regulated in the low-dose group at all time points and in the medium-dose group only at 7, 15 days after operation; the expression of Ang-2 protein was up-regulated and the neovascularization count was more in the low-dose and medium-dose group at all time points, and the neurological deficit score was lower in the low-dose group at 3 days after operation and were all lower in the medium-dose group at all time points, and all the above difference had statistical significance. Moreover, there were no significant difference in hematoma volume at 7 and 15 days after operation between the low-dose and medium-dose dl-3-n-butylphthalide groups and no-treatment model group. Conclusions Dl-3-n-butylphthalide can significantly alleviate neurological deficit in cerebral hemorrhage rats, with no increase in the risk of hematoma enlargement. The mechanism of dl-3-n-butylphthalide may be related to up-regulating the expressions of VEGF and Ang-2 protein and increasing the blood vessel density around hematoma.关键词
脑出血/血管内皮生长因子/血管生成素-2/丁基苯酞Key words
Intracerebral hemorrhage/Vascular endothelial growth factor/Angiopoietin-2/Dl-3-n-butylphthalide引用本文复制引用
涂鄂文,刘秋庭,谭莉,曾艳香,陈琼..丁基苯酞对脑出血大鼠血管内皮生长因子、血管生成素-2蛋白表达及血管新生的影响[J].中国卒中杂志,2019,14(12):1225-1231,7.基金项目
湖南省发改委科研项目(湘改高技[2012] 1493号发) （湘改高技[2012] 1493号发）
