数字中医药（英文）2019，Vol.2Issue(4)：207-218,12.DOI:10.1016/j.dcmed.2020.01.002
人参皂苷Rgl对慢性束缚应激大鼠模型的抗抑郁作用
Antidepressant-like Effects of Ginsenoside Rgl in the Chronic Restraint Stress-induced Rat Model
摘要
Abstract
Objective To investigate the ameliorating effect of ginsenoside Rg1 on the depression-like behaviors induced by chronic restraint stress (CRS) in rats and the underlying mechanisms. Methods Forty male Wistar rats were divided into 4 groups according to their baseline sucrose preference: control group, model group, and Rg1-treated groups (5 and 10 mg/kg). Except for control group, the groups were exposed to CRS (6 h/day) for 28 days. All drugs were intraperitoneally administered once daily to CRS rats after restraint stress for 14 days. The behavioral tests were carried out via the open field test (OFT), sucrose preference test (SPT), forced swim test (FST), and the Morris water maze (MWM) 4 weeks following CRS induction. The levels of serum corticosterone (CORT) and the activities of the antioxidant defense biomarkers (SOD, MDA and GSH-x) in the prefrontal cortex (PFC) were analyzed using commercial ELISA kits. The levels of the neurotransmitter (5-HT, 5-HIAA, Ach, NE, GABA and Glu) in the PFC were measured by ultra-performance liquid chromatography tandem mass spectrometry. The protein expression of BDNF, Trkb, Bax and Bcl-2 in the PFC was detected by western blotting. Results Owing to increased sucrose consumption in the SPT, decreased immobility time in the FST, and the improved cognitive performance in MWM, chronic treatment with Ginsenoside Rg1 was found to significantly attenuate depression-like behaviors (anhedonia, behavioral despair and poor spatial memory) in rats. Moreover, CRS exposure caused evident alterations in the levels of the neurotransmitters (5-HT, 5-HIAA, Ach, GABA and Glu) and the activities of the antioxidant defense biomarkers (SOD, MDA and GSH-x) in the PFC and the levels of corticosterone in serum. However, Ginsenoside Rg1 treatment could restore these levels to normal values. Additionally, Ginsenoside Rg1 treatment significantly reverted the decreased expression of BDNF, Trkb and Bcl-2 and the increased expression of Bax in the PFC of CRS rats. Conclusions Ginsenoside Rg1 could attenuate the CRS-induced depression-like behaviors, in part, by regulating neurotransmitter levels and HPA function, antagonizing oxidative stress and apoptosis, and restoring BDNF-TrkB signaling in PFC. Altogether, our results provide a novel basis regarding the potential therapeutic effects of Rg1 on depression.关键词
人参皂苷Rg1/抑郁症/慢性束缚应激/神经递质/氧化应激/BDNFKey words
Ginsenosides Rgl/Depression/Chronic restraint stress/Neurotransmitter/Oxidative stress/BDNF引用本文复制引用
姜宁,吕静薇,王海霞,黄红,王琼,陈善广,曲丽娜,Alberto Carlos Pires Dias,刘新民..人参皂苷Rgl对慢性束缚应激大鼠模型的抗抑郁作用[J].数字中医药（英文）,2019,2(4):207-218,12.基金项目
We thank for the funding support from the opening foundation of the State Key Laboratory of Space Medicine Fundamentals and Application,Chinese Astronaut Research and Training Center (No.SYFD150051808K),National Natural Science Foundation of China (No.81773930) and Development of animal model on human diseases (No.2016-I2M-2-006). （No.SYFD150051808K）
