数字中医药（英文）2019，Vol.2Issue(4)：219-226,8.DOI:10.1016/j.dcmed.2020.01.003
益气解毒方水提物通过线粒体途径诱导鼻咽癌干细胞凋亡
Yi Qi Jie Du Decoction Inhibits Proliferation and Induces Apoptosis of Nasopharyngeal Carcinoma Stem Cells Through Mitochondrial Apoptosis Pathway
摘要
Abstract
Objective To assess the effects of Yi Qi Jie Du Decoction (YQJDD) on nasopharyngeal carcinoma stem cells (NPC-SCs) and investigate the underlying mechanism. Methods NPC-SCs were collected in serum-free culture system and identified by the sphere formation assay. The effect of YQJDD on the proliferation of NPC-SCs was detected using the Cell Counting Kit-8 assay. The change of mitochondrial membrane potential (ΔΨm) in NPC-SCs following treatment with YQJDD was investigated by JC-1 staining. The levels of apoptosis-associated proteins were examined using western blot analysis. Results YQJDD significantly inhibited the proliferation of NPC-SCs in dose- and time-dependent manners. JC-1 staining revealed that YQJDD lowered the ΔΨm of NPC-SCs in a time-dependent manner. After treatment with YQJDD, the expression of cleaved caspase-3, -7 and -9, cleaved poly-ADP ribose polymerase, P21 and P53 were increased, while the expression of Survivin in NPC-SCs was decreased. However, the expression of cleaved caspase-8 remained nearly unchanged. Conclusions YQJDD inhibits the growth and induces apoptosis of NPC-SCs via an intrinsic apoptosis pathway.关键词
鼻咽癌/益气解毒方/细胞凋亡/肿瘤干细胞/线粒体凋亡途径Key words
Nasopharyngeal carcinoma (NPC)/Yi Qi Jie Du Decoction (YQJDD)/Apoptosis/Cancer stem cells/Mitochondrial apoptosis pathway引用本文复制引用
周芳亮a,b,何兰a,何丹a,王贤文a,史红健a,何迎春a,b,CAO De-Liangc..益气解毒方水提物通过线粒体途径诱导鼻咽癌干细胞凋亡[J].数字中医药（英文）,2019,2(4):219-226,8.基金项目
We thank for the funding support from the National Natural Science Foundation of China (No.81573721,No.81874408 and No.81973914),the Natural Science Foundation of Hunan (No.2017JJ3246 and No.2019JJ40216) and Excellent Youth Fund of Hunan Provincial Education Department (No.19B439). （No.81573721,No.81874408 and No.81973914）
