中国组织工程研究2020，Vol.24Issue(13)：2087-2094,8.DOI:10.3969/j.issn.2095-4344.2063
间充质干细胞源性外泌体的检测方法
Detection methods of mesenchymal stem cells-derived exosomes
摘要
Abstract
BACKGROUND:Mesenchymal stem cells-derived exosomes have been applied in the diagnosis and treatment of various diseases due to its many virtues. Therefore, its accurate and effective detection with low cost is critical for disease diagnosis and treatment. OBJECTIVE: To introduce the detection methods and newest progress of mesenchymal stem cells-derived exosomes. METHODS: WanFang, Baidu Scholar, VIP, CNKI, PubMed, Web of Science, Sinomed, Embase, Cochrane and Web of Knowledge databases were retrieved for the articles concerning the detection methods of mesenchymal stem cells-derived exosomes published between June 1997 and June 2019. The keywords were "mesenchymal stem cells, exosomes, detection methods" in Chinese and English, respectively. The duplicated and poorly correlated articles were excluded, and finally 60 eligible articles were included for analysis. RESULTS AND CONCLUSION: (1) The definition and biological characteristics of mesenchymal stem cells are summarized, and mesenchymal stem cells have been found to treat diseases by paracrine approach. (2) The definition and biological characteristics of mesenchymal stem cells-derived exosomes and its potential clinical application are summarized, including immunoregulation in vivo and promotion of tissue regeneration. (3) The commonly used detection methods of mesenchymal stem cells-derived exosomes and the newest progress are reviewed. (4) This review provides experimental basis for the clinical application of mesenchymal stem cells-derived exosomes regarding disease treatment and tissue repair.关键词
间充质干细胞/外泌体/检测方法/间充质干细胞源性外泌体/表征/组织修复Key words
mesenchymal stem cells/exosomes/detection methods/mesenchymal stem cell-derived exosomes/characterization/tissue repair分类
医药卫生引用本文复制引用
杨沁馨,王达利,卫雪..间充质干细胞源性外泌体的检测方法[J].中国组织工程研究,2020,24(13):2087-2094,8.基金项目
国家自然科学基金(81871570),项目负责人:王达利 （81871570）
