林业科学2020，Vol.56Issue(1)：38-54,17.DOI:10.11707/j.1001-7488.20200105
转录组与代谢组联合解析红花槭叶片中花青素苷变化机制
Combined Transcriptomic and Metabolomic Analysis Reveals Mechanism of Anthocyanin Changes in Red Maple(Acer rubrum) Leaves
摘要
Abstract
[Objective] The autumn leaf color of red maple (Acer rubrum) changed from green to red or yellow, which was closely related to the content of anthocyanin in leaves. With the increasing demand for directional breeding of garden plants，this paper aims to reveal the mechanism of anthocyanin biosynthesis in red maple and provide theoretical basis for directional improvement of its leaf color.[Method] In order to analyze the changes of anthocyanin metabolite accumulation and gene expression level，this study employed green leaves，red leaves and yellow leaves of red maple color mutant as experimental materials，and applied UHPLC-QE-MS and high-throughput RNA sequencing method for metabolome and transcriptome analysis，respectively. [Result] 1)In the comparison group of red-green leaves，yellow-green leaves，red- yellow leaves，1 377，1 793 and 1 098 differential accumulated metabolites were detected respectively under the positive ion mode，789，699，677 differential accumulated metabolites were detected respectively under the negative ion mode: in red leaves，the content of cyanidin，pelargonidin and delphinidin and their derivatives increased significantly compared with green leaves; in yellow leaves，the content of cyanidin，delphinidin and its derivatives increased，while the pelargonidin and its derivatives decreased compared with green leaves. 2) In the above-mentioned three comparison groups，transcriptome sequencing detected 28 536，43 017，27 110 differentially expressed genes，respectively: compared with green leaves，89.5% gene expression in the anthocyanidin synthesis pathway of red leaves increased，66.7% gene expression in the anthocyanidin synthesis pathway of yellow leaves increased. 3) In the anthocyanidin biosynthesis of red maple, there were 29 differentially accumulated metabolites and 48 differentially expressed genes. 4) Network interaction analysis of differential metabolites and genes showed that CHS2，CHS7, CHS8，F3H1，F3H5，F3H7, F3H8，F3H10，F3' H2，LAR，FLSl，FL S2，UFGT4 negatively regulate the synthesis of pelargonidin derivatives and delphinidin derivatives，and UFGT5 positively regulate the synthesis of cyanidin derivatives. [Conclusion]When red maple leaves change color in autumn，the expression of a large number of genes in the anthocyanin pathway is up-regulated，and the content of cyanidin 3-(6"-acetyl-galactoside) and cyanidin 3-arabinoside increased，which is one of the main reasons why leaves change the color.关键词
红花槭/花青素苷/叶色/代谢组/转录组Key words
Acer rubrum/anthocyanin/colored leaves/metabolome/transcriptome分类
农业科技引用本文复制引用
陆小雨,陈竹,唐菲,傅松玲,任杰..转录组与代谢组联合解析红花槭叶片中花青素苷变化机制[J].林业科学,2020,56(1):38-54,17.基金项目
国家自然科学基金项目(31600543) （31600543）
安徽省自然科学基金项目(1708085MC57) （1708085MC57）
安徽省农业科学院团队项目(2019YL055). （2019YL055）
