生命科学研究2020，Vol.24Issue(1)：7-14,8.DOI:10.16605/j.cnki.1007-7847.2020.01.002
拟南芥响应高钙的miRNAs的筛选与鉴定
Identification and Characterization of miRNAs in Response to High Calcium in Arabidopsis
摘要
Abstract
Karst soils with high calcium levels restrict plant growth,which then affects crop yield and quality.At present,little research has been done on the mechanisms of plants responding to high calcium stress.miRNAs play important roles in plant responses to biotic and abiotic stress.Herein,twelve high calcium-responsive A rabidopsis miRNAs were identified using high-throughput sequencing and strictly standardized statistical analysis,and their target genes were predicted and analyzed.Real-time quantitative PCR analysis confirmed that expressions of most of the differentially expressed miRNAs were negatively correlated with those of their target genes.This study provides a theoretical basis for revealing the mechanisms of plants responding to high calcium stress and the roles of miRNAs in this process.关键词
高钙/拟南芥/miRNA/靶基因Key words
high calcium/Arabidopsis/miRNA/target gene分类
农业科技引用本文复制引用
李菲,石天龙,龚记熠,张习敏,汤晓辛,乙引..拟南芥响应高钙的miRNAs的筛选与鉴定[J].生命科学研究,2020,24(1):7-14,8.基金项目
国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心项目(U1812401)Joint Project of National Natural Science Fund Committee and Guizhou Karst Research Center (U1812401) （U1812401）
