中国水稻科学2020，Vol.34Issue(2)：125-134,10.DOI:10.16819/j.1001-7216.2020.9125
利用剩余杂合体衍生的近等基因系精细定位水稻粒长微效QTL qGL1.1
Fine Mapping of qGL1.1, a Minor QTL for Grain Length, Using Near Isogenic Lines Derived from Residual Heterozygotes in Rice
摘要
Abstract
[Objective] The objective of this study is to fine-map qTGWl.l b which was previously mapped in a 521.8-kb region on the long arm of rice chromosome 1. [Method] In this study, two BC2F9 plants carrying qTGWl.l and qTGW1.2, respectively, was crossed and produced an F4 population. Three plants carrying sequential heterozygous segments in the interval Wn28826-RM1231 were selected and selfed to develop three sets of near isogenic lines (NILs) comprising the two homozygous genotypes. The NILs in F5:6 were planted in Hangzhou in 2017 and the 1000-grain weight, grain length and grain width were measured. The effect of qTGW1.1 b was validated by two-way analysis of variance (ANOVA) using SAS program. Then six new sequential residual heterozygotes carrying smaller heterozygous segments overlapped in the interval Wn28826-RM1231 were identified. Six sets of pairwise NILs in F8:9 were planted in Lingshui in 2018 and the three traits were measured. Phenotypic differences between the two homozygous genotypic groups were analyzed using ANOVA. [Results] The allelic direction of qTGWl.l b remained consistent and the genetic effects were stable in the two trials. The Milyang 46 allele could significantly increase grain length and 1000-grain weight by 0.027 mm and 0.17 g with the contribution up to 27.12% and 19.09%, respectively. [Conclusion] Considering that qTGWl.l b stably showed significant effect to grain length but not to grain width in both previous and this study, qTGWl.l b was renamed as qGLl.l. As a result, qGLl.l was delimited into a 76.8-kb region flanked by Wn29077 and Wn29154 based on comparing of the segregating regions among the six sets of NILs.关键词
水稻/近等基因系/微效作用/粒长/精细定位Key words
rice/near isogenic line/minor effect/grain length/fine mapping分类
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李盼盼1,朱玉君1,郭梁2,庄杰云1,樊叶杨1*..利用剩余杂合体衍生的近等基因系精细定位水稻粒长微效QTL qGL1.1[J].中国水稻科学,2020,34(2):125-134,10.基金项目
国家重点研发计划资助项目(2017YFD0100305) （2017YFD0100305）
中央公益性科研院所基本科研业务费专项(2017RG001-2). （2017RG001-2）
