中国神经再生研究（英文版）2020，Vol.15Issue(10)：1962-1968,7.DOI:10.4103/1673-5374.280328
Expression profiles of long noncoding RNAs in retinopathy of prematurity
摘要
Abstract
Long noncoding RNA (lncRNA) regulates the proliferation and migration of human retinal endothelial cells, as well as retinal neovas-cularization in diabetic retinopathy. Based on similarities between the pathogenesis of retinopathy of prematurity (ROP) and diabetic retinopathy, lncRNA may also play a role in ROP. Seven-day-old mice were administered 75 ± 2% oxygen for 5 days and normoxic air for another 5 days to establish a ROP model. Expression of lncRNA and mRNA in the retinal tissue of mice was detected by high-throughput sequencing technology, and biological functions of the resulted differentially expressed RNAs were evaluated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. The results showed that compared with the control group, 57 lncRNAs were differentially expressed, including 43 upregulated and 14 downregulated, in the retinal tissue of ROP mice. Compared with control mice, 42 mRNAs were differentially expressed in the retinal tissue of ROP mice, including 24 upregulated and 18 downregulated mRNAs. Differentially expressed genes were involved in ocular development and related metabolic pathways. The differentially expressed lncRNAs may regulate ROP in mice via microRNAs and multiple signaling pathways. Our results revealed that these differentially expressed lncRNAs may be therapeutic targets for ROP treatment. This study was approved by the Medical Ethics Committee of Shengjing Hospital of China Medical University on February 25, 2016 (approval No. 2016PS074K).关键词
bioinformatics/gene therapy/long noncoding RNA/microglial/neurovascular disease/optic neuropathy/retinal development/retinal neovascularization/retinopathy of prematurity/signaling pathwaysKey words
bioinformatics/gene therapy/long noncoding RNA/microglial/neurovascular disease/optic neuropathy/retinal development/retinal neovascularization/retinopathy of prematurity/signaling pathways引用本文复制引用
Yue Wang,Xue Wang,Yuan Ma,Yue-Xia Wang,Yu Di..Expression profiles of long noncoding RNAs in retinopathy of prematurity[J].中国神经再生研究（英文版）,2020,15(10):1962-1968,7.基金项目
The work was supported by the National Natural Science Foundation of China, No. 81600747 (to YD) and Startup Foundation for Doctors of Liaoning Province, China, No. 201501020 (to YD). （to YD）
