南方医科大学学报2020，Vol.40Issue(5)：609-614,6.DOI:10.12122/j.issn.1673-4254.2020.05.01
神经性厌食症大鼠伏隔核与延髓头端腹外侧区的神经通路联系
Neural pathway between the nucleus accumbens and the rostral ventrolateral medulla in a rat model of anorexia nervosa
摘要
Abstract
Objective To investigate the potential neural pathway connecting the nucleus accumbens (NAc) and the rostral ventrolateral medulla (RVLM), and whether the pathway participates in the regulation of cardiovascular function in a model rat of anorexia nervosa (AN). Methods Rat models of AN were established by allowing voluntary activity in a running wheel with restricted feeding, with the rats having free access to normal chow without exercise as the control group. FluoroGold (FG) retrograde tracing method and multi-channel simultaneous recording technique were used to explore the possible pathway between the NAc and the RVLM. Results The rats in AN group exhibited significantly reduced systolic blood pressure (SBP), mean arterial pressure (MAP) and heart rate (HR) with significantly increased discharge frequency of RVLM neurons in comparison with the control rats. After the injection of FG into the RVLM, retrograde labeled neurons were observed in the NAc of the rats in both the normal control and AN groups. In both groups, SBP and HR were significantly decreased in response to 400 μA electrical stimulation of the NAc accompanied by an obvious increase in the discharge frequency of the RVLM neurons;the diastolic blood pressure (DBP) and MAP were significantly lower in AN model rats than in the normal rats in response to the stimulation. Conclusions We successfully established a rat model of AN via hyperactivity and restricted feeding and confirm the presence of a neural pathway connecting the NAc and the RVLM. This pathway might participate in the regulation of cardiovascular function in AN model rats.关键词
神经性厌食症/延髓头端腹外侧区/伏隔核/心血管功能Key words
anorexia nervosa/rostral ventrolateral medulla/nucleus accumbens/cardiovascular function引用本文复制引用
魏雯雯,吴琼波,郑超,汪萌芽,张环环..神经性厌食症大鼠伏隔核与延髓头端腹外侧区的神经通路联系[J].南方医科大学学报,2020,40(5):609-614,6.基金项目
安徽高校自然科学研究项目(KJ2018A0266,KJ2019A0411) （KJ2018A0266,KJ2019A0411）
国家自然科学基金(31271155,31200828) （31271155,31200828）
皖南医学院大学生科研基金(WK2019S57)Supported by Natural Science Research Project of Colleges and Universities in Anhui Province (KJ2018A0266, KJ2019A0411), National Natural Science Foundation of China (31271155, 31200828) and Student Research Fund Project of Wannan Medical College (WK2019S57). （WK2019S57）
