广西植物2019，Vol.39Issue(12)：1681-1690,10.DOI:10.11931/guihaia.gxzw201808022
没食子酸对水稻细菌性条斑病菌细胞结构的影响
Effects of gallic acid on cell structure of Xanthomonas oryzae pv.oryzicola
摘要
Abstract
Gallic acid (GA) is a phenolic compound,and presents various biological activities in plants.Our previous experiments demonstrated a relatively strong inhibitory effect of GA on Xanthomonas oryzae pv.oryzicola (Xoc).In order to elucidate the effects of GA on the cell structure and membrane permeability of the pathogen,morphological structures of bacteria treated with GA were observed by electron microscopy,the integrity and the permeabilities of membrane of Xoc were investigated by determining the release from cells of materials that absorb at 260 nm,and changes in the fluorescence of cells treated with the fluorescein diacetate(FDA) and lactate dehydrogenase(LDH) activity.After treating with 200 μg · mL-1of GA,many pits and irregular vesicles were found on the cell surface under electron microscopy,indicating that GA could damage the cell walls of Xoc.The electrical conductivity from Xoc suspensions 24 h after GA treatment was 135.48 μS · cm-1(that of control was 127.85 μS · cm-1),and the fluorescence intensity of Xoc suspensions 2 h after GA decreased by 58.10％,indicating that cells leaked electrolytes and cytosolic contents.Meanwhile,the activities of LDH in bacterial suspensions treated with GA also increased,suggesting that GA could damage the structure of the bacterial cell membrane.In addition,the absorbance at 260 nm from Xoc suspensions was 1.004(that of control was 0.018),indicating that GA could negatively affect Xoc cell wall integrity.These results indicate that GA can not only alter the permeability of the cell membrane of Xoc,but also can impact the integrity of cell membrane.关键词
水稻细菌性条斑病菌/没食子酸/细胞结构/膜通透性/抑菌机制Key words
Xanthomonas oryzae pv.oryzicola/gallic acid/cell structure/membrane permeability/bacteriostasis mechanism分类
农业科技引用本文复制引用
张锡娇,黎芳靖,袁高庆,魏昌英,林纬,黎起秦..没食子酸对水稻细菌性条斑病菌细胞结构的影响[J].广西植物,2019,39(12):1681-1690,10.基金项目
广西自然科学基金(2014GXNSFAA118073) （2014GXNSFAA118073）
