广西医科大学学报2020，Vol.37Issue(4)：559-568,10.DOI:10.16190/j.cnki.45-1211/r.2020.04.001
MiR-221-3p调控c-Fos/beclin-1对大鼠冠状动脉微栓塞后心肌细胞自噬的影响
Effects of miR-221-3p regulating c-Fos/beclin-1 signaling pathway on autophagy of myocardial cells in rats after coronary microembolization
摘要
Abstract
Objective:To investigate the effect of miR-221-3p mediated c-Fos/beclin-1 signaling pathway on myocardial autophagy in rats after coronary micmembolization(CME).Methods:A total of 24 SD rats were randomly divided into the Sham group,the CME group,the CME + AAV9-miR-221-3p shRNA group (CME + shRNA group),and the CME + AAV9-Control shRNA group (CME + NC group),with 6 rats in each group.CME model was established by injecting polyethylene microspheres (42 μmn)into the left ventricle,and an equal amount of saline was used in the Sham group.Cardiac ultrasound and cardiac troponin Ⅰ(cTn-Ⅰ)were measured in each group after 9 h operation.Tissue samples were taken for hematoxylin-basic fuchsin-picric acid (HBFP),transmission electron microscope (TEM),immunofluorescence,fluorescent quantitation polymerase chain reaction(RT-qPCR),and Western blot to evaluate the effect of miR-221-3p mediated c-Fos/beclin-1 signaling pathway on CME's function,shape,and molecular aspect.Results:Compared with the Sham group,the expression of miR-221-3p in cardiomyocytes was increased,cardiac function was suppressed,cTn-Ⅰ and myocardial microinfarction areas were significantly increased,and the protein levels of c-Fos,beclin-1,and LC3-Ⅱ were decreased of the CME group (P＜0.05).Compared with the CME group,cTn-Ⅰ and myocardial infarction areas were significantly reduced after miR-221-3p was down-regulated (P＜ 0.05);protein levels of c-Fos,beclin-1,and LC3-Ⅱ were increased (P＜0.05).TEM image showed that double membranes of autophagic vacuoles were increased,autophagy was partially recovered,myocardial damage was reduced,and cardiac function was improved.Conclusion:Inhibition of miR-221-3p can improve cardiac function in rats after CME,which may regulate myocardial autophagy and participate in CME-induced myocardial injury via c-Fos/beclin-1 pathway.关键词
冠状动脉微栓塞/miR-221-3p/c-Fos/beclin-1/自噬Key words
coronary microembolization/miR-221-3p/c-Fos/beclin-1/autophagy分类
医药卫生引用本文复制引用
吴文豪,李浪,郑静,龙曼云,王现涛,孙羽涵,苏波..MiR-221-3p调控c-Fos/beclin-1对大鼠冠状动脉微栓塞后心肌细胞自噬的影响[J].广西医科大学学报,2020,37(4):559-568,10.基金项目
This study was supported by grants from the National Natural Science Foundation of China (No.81770346). （No.81770346）
