南京林业大学学报（自然科学版）2020，Vol.44Issue(3)：33-40,8.DOI:10.3969/j.issn.1000-2006.201906033
矢竹地下茎节间生长的解剖学和转录组研究
Anatomical and transcriptomic analysis of bamboo rhizome internode growth
摘要
Abstract
[Objective]The underground rhizome is the main stem of the running bamboo forest. However,little is knownabout its development,such as its elongation that is important for bamboo forest spread. In the present study,we aim to explore the cellular and transcriptomic features of rhizome internode elongation of Pseudosasa japonicas.[Method]Cellu?lar observation was completed via paraffin sectioning,analysis of the transcriptomic profile via next?generation sequenc?ing technology,and visualization,as well as enrichment analysis of differentially expressed genes,via MapMan.[Re?sult]Through the morphological analysis of P. japonica's rhizome elongation,we discovered that the elongation was di?rectly promoted by the growth of -14 young internodes in the rhizome shoot. The anatomical analysis results further re?vealed that internodes with lengths lower than 0.4 cm had strong cell division ability,and distinctive cell elongation was found in a 1.0 cm long internode. Long and short parenchymal cells were apparent in the 1.0 cm internode and its vascu?lar tissue such as vessel cells and fiber cells were much longer and more developed than in the 0.4 cm internode. It was also observed that pith tissue broke to form the pith cavity in the 1.0 cm internode. The RNA?Seq analysis between 0.4 and 1.0 cm internodes revealed a transition of transcriptomic activities. This included a transition from cell division and basic cellular metabolism in the 0.4 cm internode to active cell growth processes such as cell wall biosynthesis and sec?ondary metabolism,and in the 1.0 cm internode to Programmed Cell Death processes such as ethylene signal pathways and(reactive oxygen species,ROS)burst genes.[Conclusion]There is a transition of cell growth processes from cell di?vision to cell elongation during P. japonica's rhizome internode elongation. Additionally,developmental events such as pith cavity formation exist in the 1.0 cm internode,which might be due to upregulation of genes related to cell growth and Programmed Cell Death.关键词
节间生长/快速生长/转录组测序/活性氧/细胞壁/地下茎Key words
internode growth/fast growth/RNA⁃Seq/reactive oxygen species(ROS)/cell wall/rhizome分类
农业科技引用本文复制引用
向钰,丁雨龙,张春霞,魏强..矢竹地下茎节间生长的解剖学和转录组研究[J].南京林业大学学报（自然科学版）,2020,44(3):33-40,8.基金项目
"十二五"农村领域国家科技计划(2015BAD04B0305) （2015BAD04B0305）
国家自然科学基金项目( 31670602) （ 31670602）
江苏省高校"青蓝工程"资助项目 （）
江苏高校优势学科建设工程资助项目(PAPD). （PAPD）
