南京林业大学学报（自然科学版）2020，Vol.44Issue(3)：95-104,10.DOI:10.3969/j.issn.1000-2006.201908022
枫香根际解有机磷细菌筛选及其促生效应
Screening of indigenous phosphate?solubilizing bacteria from Liquidambar formosa?na Hance rhizosphere and its potential applications for improving plant growth
摘要
Abstract
[Objective]Isolate and identify efficient phosphate solubilizing bacteria(PSB)from Liquidambar formosanaHance rhizosphere soil and investigate the application in phosphorus release.[Method]the PSB strains were graded based on morphological and physiological?biochemical characteristics,microbial identification system(MIDI),BIO?LOG analysis and 16S rRNA identification methods. The effect of these PSB strains(JX7,JX18,JX21 and WH10)on plant growth was evaluated by determining the minor and major mineral contents of L. formosana leaves using inductive?ly?coupled plasma atomic emission spectrometry.[Result]Isolates JX7,JX18,WH10 and JX21 were closely related to Variovorax paradoxus,Bacillus cereus,B. mycoides and B.cereus,respectively. The JX21 showed the highest content of soluble phosphate was(9.35 ± 0.89)mg/L,followed by JX7[(6.63±0.09)mg/L],WH10[(6.16 ± 0.12 )mg/L]andJX18[(5.32 ± 0.07 )mg/L],all the maximum phosphate was fermented after 72 h of incubation. Phosphate?solubilizing bacteria(JX7,WH10,JX18 and JX21)selected from L. formosana rhizosphere showed a significantly improvement in shoot height and ground diameter of L. formosana(P<0.05). As well as,these selected strains promoted L. formosana in taking up mineral elements such as phosphorus,magnesium,calcium and zinc under greenhouse conditions.[Conclu?sion]The isolates showed good phosphate?solubilizing activity and had potential for use as biofertilizer agents. Further study is needed to evaluate beneficial traits to develop commercial applications.关键词
枫香/植物促生菌/植物营养素/解磷菌/生物肥料Key words
Liquidambar formosana Hance/plant growth promoting bacteria/plant nutrients/phosphate‑solubilizing bacteria/biofertilizer分类
农业科技引用本文复制引用
宋娟,徐国芳,赵邢,姚尧,杨学祥,唐荣林,崔家旺,陈凤毛,任嘉红..枫香根际解有机磷细菌筛选及其促生效应[J].南京林业大学学报（自然科学版）,2020,44(3):95-104,10.基金项目
国家自然科学基金项目(31100471) （31100471）
国家重点研发计划(2017YFD0600104) （2017YFD0600104）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
江苏省林业科技创新与推广项目(LYKJ[2017]20). （LYKJ[2017]20）
