广西医科大学学报2020，Vol.37Issue(5)：777-783,7.DOI:10.16190/j.cnki.45-1211/r.2020.05.001
病毒性心肌炎小鼠心肌组织中集聚蛋白的变化
Changes of myocardial agrin in mice with coxsackievirus B3-induced myocarditis
覃琳 1黄凯 1李勇 2黄炎兰 1伍伟锋1
作者信息
- 1. 广西医科大学第一附属医院心血管内科,南宁530021
- 2. 广西医科大学第一附属医院急诊科,南宁530021
- 折叠
摘要
Abstract
Objective:To evaluate the changes of agrin expression in the myocardium of viral myocarditis (VMC) mice induced by coxsackievirus B3 (CVB3),and to explore the role of agrin in the pathology of VMC.Methods:A total of 80 male BALB/c mice were randomly divided into a control group (phosphate-buffered saline,PBS) and an experimental group (PBS containing CVB3).Mice in each group were divided into four subgroups according to various time points (1,2,4,and 6 wks) after injection,and the hearts of the mice were harvested at these time points.Hematoxylin-eosin (HE) staining was used to examine the pathological changes in the myocardium,and immunohistochemistry was used to investigate the agrin expression in myocardial tissue.In addition,semi-quantitative reverse transcription polymerase chain reaction and western blotting were used to detect the expression of agrin mRNA and agrin protein,respectively.Results:HE staining indicated that the VMC mouse model was established successfully.In the experimental group,the expression of agrin,agrin mRNA,and agrin protein began to decrease in the subgroup at week 1,slightly increased at week 2,and then gradually decreased at weeks 4 and 6.The expression of agrin in all experimental subgroups was significantly lower than that in the corresponding control subgroups (P＜0.05).Conclusion:The expression of agrin is diminished in VMC mice,suggesting that agrin may be involved in the pathogenesis of VMC.关键词
病毒性心肌炎/柯萨奇病毒B3/集聚蛋白/表达Key words
viral myocarditis/coxsackievirus B3/agrin/expression分类
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覃琳,黄凯,李勇,黄炎兰,伍伟锋..病毒性心肌炎小鼠心肌组织中集聚蛋白的变化[J].广西医科大学学报,2020,37(5):777-783,7.
